Chrome Azurol S Explained

Chrome Azurol S is a histological dye used in biomedical research.^{[1] [2]}

Chrome Azural S (CAS) is a common spectrophotometric reagent for detection of certain metals like aluminum which can be toxic in excess and can contribute to people with neurodegenerative disorders.^[3] CAS is used to provide quantitative and qualitative information on molecules of interest like aluminum and siderophores.^[4] Qualitatively a color change can be observed while also allowing to quantitatively determine concentration of certain ions.

Notes and References

1. The photometric titration of Fe(III) with EDTA using tiron indicator . Berger . Selman A. . McKay . J. Brian . July 1974 . Microchimica Acta . 62 . 4 . 665–670 . 10.1007/bf01218204. 4216747 . 32908378 .
2. Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria . Alexander . D. . Zuberer . David . 1991-09-01 . Biology and Fertility of Soils . 12 . 39–45 . 10.1007/BF00369386. 25744344 .
3. Ngarisan . Noor Izyan . Ngah . Che Wan Zanariah Che Wan . Ahmad . Musa . Kuswandi . Bambang . 2014-11-01 . Optimization of polymer inclusion membranes (PIMs) preparation for immobilization of Chrome Azurol S for optical sensing of aluminum(III) . Sensors and Actuators B: Chemical . en . 203 . 465–470 . 10.1016/j.snb.2014.07.023 . 0925-4005.
4. Book: Himpsl . Stephanie D. . Mobley . Harry L. T. . Proteus mirabilis . 2019 . Siderophore Detection Using Chrome Azurol S and Cross-Feeding Assays . https://pubmed.ncbi.nlm.nih.gov/31309499/#:~:text=The%20chrome%20azurol%20S%20(CAS,change%20from%20blue%20to%20orange. . Methods in Molecular Biology . 2021 . 97–108 . 10.1007/978-1-4939-9601-8_10 . 1940-6029 . 31309499. 978-1-4939-9600-1 . 196813888 .