Chondroitin B lyase explained
| Chondroitin B lyase |
| Ec Number: | 4.2.2.19 |
| Cas Number: | 52227-83-5 |
The enzyme chondroitin B lyase catalyzes the following process:
Eliminative cleavage of dermatan sulfate containing (1→4)-β-D-hexosaminyl and (1→3)-β-D-glucurosonyl or (1→3)-α-L-iduronosyl linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groups to yield a 4,5-unsaturated dermatan-sulfate disaccharide (ΔUA-GalNAc-4S).Glossary:
This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. The systematic name of this enzyme class is chondroitin B lyase. Other names in common use include chondroitinase B, ChonB, and ChnB.
References
- Gu K, Linhardt RJ, Laliberte M, Gu K, Zimmermann J . Purification, characterization and specificity of chondroitin lyases and glycuronidase from Flavobacterium heparinum . Biochem. J. . 312 . 569 - 77 . 8526872 . 1136300 . Pt 2 . 1995 . 10.1042/bj3120569 .
- Pojasek K, Raman R, Kiley P, Venkataraman G, Sasisekharan R . 2002 . Biochemical characterization of the chondroitinase B active site . J. Biol. Chem. . 277 . 31179 - 86 . 12063249 . 10.1074/jbc.M201552200 . 34 . free .
- Pojasek K, Shriver Z, Kiley P, Venkataraman G, Sasisekharan R . 2001 . Recombinant expression, purification, and kinetic characterization of chondroitinase AC and chondroitinase B from Flavobacterium heparinum . Biochem. Biophys. Res. Commun. . 286 . 343 - 51 . 11500043 . 10.1006/bbrc.2001.5380 . 2 .
- Suzuki K, Terasaki Y, Uyeda M . 2002 . Inhibition of hyaluronidases and chondroitinases by fatty acids . J. Enzyme. Inhib. Med. Chem. . 17 . 183 - 6 . 12443044 . 10.1080/14756360290032930 . 3 .
- Ototani N, Yosizawa Z . 1979 . Purification of chondroitinase B and chondroitinase C using glycosaminoglycan-bound AH-Sepharose 4B . Carbohydr. Res. . 70 . 295 - 306 . 427837 . 10.1016/S0008-6215(00)87109-8 . 2 .
- K, Zimmermann JJ . Su H . 2000 . Isolation and Expression in Escherichia coli of cslA and cslB, Genes Coding for the Chondroitin Sulfate-Degrading Enzymes Chondroitinase AC and Chondroitinase B, Respectively, from Flavobacterium heparinum . Appl. Environ. Microbiol. . 66 . 29 - 35 . 10618199 . 10.1128/AEM.66.1.29-35.2000 . 1 . 91781 . 2000ApEnM..66...29T .
- V, Sasisekharan R, Cygler M . 2004 . The structure of chondroitin B lyase complexed with glycosaminoglycan oligosaccharides unravels a calcium-dependent catalytic machinery . J. Biol. Chem. . 279 . 32882 - 96 . 15155751 . 4135467 . 10.1074/jbc.M403421200 . 31 . free .
- Li Y, Matte A, Su H, Cygler M . 1999 . Crystallization and preliminary X-ray analysis of chondroitinase B from Flavobacterium heparinum . Acta Crystallogr. D . 55 . 1055 - 7 . 10216304 . 10.1107/S0907444999002097 . Pt 5 . 1999AcCrD..55.1055L .
- Huang W, Matte A, Li Y, Kim YS, Linhardt RJ, Su H, Cygler M . 1999 . Crystal structure of chondroitinase B from Flavobacterium heparinum and its complex with a disaccharide product at 1.7 A resolution . J. Mol. Biol. . 294 . 1257 - 69 . 10600383 . 10.1006/jmbi.1999.3292 . 5 .
- Lauder RM . 2005 . Characterization of oligosaccharides from the chondroitin/dermatan sulfates. 1H-NMR and 13C-NMR studies of reduced trisaccharides and hexasaccharides . FEBS J. . 272 . 6276 - 86 . 16336265 . 10.1111/j.1742-4658.2005.05009.x . Nieduszynski . IA . Giannopoulos . M . Weeks . SD . Sadler . IH . Lauder . RM . 24 . free .
