Chondroitin-sulfate-ABC exolyase explained

Chondroitin-sulfate-ABC exolyase
Ec Number:	4.2.2.21

The enzyme chondroitin-sulfate-ABC exolyase catalyzes the following process:

Exolytic removal of Δ⁴-unsaturated disaccharide residues from the non-reducing ends of both polymeric chondroitin/dermatan sulfates and their oligosaccharide fragments.

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. The systematic name of this enzyme class is chondroitin-sulfate-ABC exolyase. Other names in common use include chondroitinase (ambiguous), chondroitin ABC eliminase (ambiguous), chondroitinase ABC (ambiguous), chondroitin ABC lyase (ambiguous), chondroitin sulfate ABC lyase (ambiguous), ChS ABC lyase (ambiguous), chondroitin sulfate ABC exoeliminase, chondroitin sulfate ABC exolyase, and ChS ABC lyase II.

References

• Yamagata T, Saito H, Habuchi O, Suzuki S . 1968 . Purification and properties of bacterial chondroitinases and chondrosulfatases . J. Biol. Chem. . 243 . 1523 - 35 . 5647268 . 7 .
• Saito H, Yamagata T, Suzuki S . 1968 . Enzymatic methods for the determination of small quantities of isomeric chondroitin sulfates . J. Biol. Chem. . 243 . 1536 - 42 . 4231029 . 7 .
• Suzuki S, Saito H, Yamagata T, Anno K, Seno N, Kawai Y, Furuhashi T . 1968 . Formation of three types of disulfated disaccharides from chondroitin sulfates by chondroitinase digestion . J. Biol. Chem. . 243 . 1543 - 50 . 5647269 . 7 .
• Suzuki S . 1997 . Two distinct chondroitin sulfate ABC lyases. An endoeliminase yielding tetrasaccharides and an exoeliminase preferentially acting on oligosaccharides . J. Biol. Chem. . 272 . 9123 - 30 . 9083041 . 10.1074/jbc.272.14.9123 . Hashimoto . N . Mochizuki . H . Kato . F . Makiguchi . Y . Horie . K . Suzuki . S . 14 . free .
• Lauder RM . 2005 . Characterization of oligosaccharides from the chondroitin/dermatan sulfates. 1H-NMR and 13C-NMR studies of reduced trisaccharides and hexasaccharides . FEBS J. . 272 . 6276 - 86 . 16336265 . 10.1111/j.1742-4658.2005.05009.x . Nieduszynski . IA . Giannopoulos . M . Weeks . SD . Sadler . IH . Lauder . RM . 24 . free .