Chondroitin-sulfate-ABC endolyase explained

Chondroitin-sulfate-ABC endolyase
Ec Number:	4.2.2.20

The enzyme chondroitin-sulfate-ABC endolyase catalyzes the following process:

Endolytic cleavage of (1→4)-β-galactosaminic bonds between N-acetylgalactosamine and either D-glucuronic acid or L-iduronic acid to produce a mixture of Δ⁴-unsaturated oligosaccharides of different sizes that are ultimately degraded to Δ⁴-unsaturated tetra- and disaccharides

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. The systematic name of this enzyme class is chondroitin-sulfate-ABC endolyase. Other names in common use include chondroitinase (ambiguous), chondroitin ABC eliminase (ambiguous), chondroitinase ABC (ambiguous), chondroitin ABC lyase (ambiguous), chondroitin sulfate ABC lyase (ambiguous), ChS ABC lyase (ambiguous), chondroitin sulfate ABC endoeliminase, chondroitin sulfate ABC endolyase, and ChS ABC lyase I.

References

• Yamagata T, Saito H, Habuchi O, Suzuki S . 1968 . Purification and properties of bacterial chondroitinases and chondrosulfatases . J. Biol. Chem. . 243 . 1523 - 35 . 5647268 . 7 .
• Saito H, Yamagata T, Suzuki S . 1968 . Enzymatic methods for the determination of small quantities of isomeric chondroitin sulfates . J. Biol. Chem. . 243 . 1536 - 42 . 4231029 . 7 .
• Suzuki S, Saito H, Yamagata T, Anno K, Seno N, Kawai Y, Furuhashi T . 1968 . Formation of three types of disulfated disaccharides from chondroitin sulfates by chondroitinase digestion . J. Biol. Chem. . 243 . 1543 - 50 . 5647269 . 7 .
• Suzuki S . 1997 . Two distinct chondroitin sulfate ABC lyases. An endoeliminase yielding tetrasaccharides and an exoeliminase preferentially acting on oligosaccharides . J. Biol. Chem. . 272 . 9123 - 30 . 9083041 . 10.1074/jbc.272.14.9123 . Hashimoto . N . Mochizuki . H . Kato . F . Makiguchi . Y . Horie . K . Suzuki . S . 14 . free .
• Lauder RM . 2005 . Characterization of oligosaccharides from the chondroitin/dermatan sulfates. 1H-NMR and 13C-NMR studies of reduced trisaccharides and hexasaccharides . FEBS J. . 272 . 6276 - 86 . 16336265 . 10.1111/j.1742-4658.2005.05009.x . Nieduszynski . IA . Giannopoulos . M . Weeks . SD . Sadler . IH . Lauder . RM . 24 . free .