Chitin disaccharide deacetylase explained

Chitin disaccharide deacetylase
Ec Number:	3.5.1.105

Chitin disaccharide deacetylase (chitobiose amidohydolase, COD, chitin oligosaccharide deacetylase, chitin oligosaccharide amidohydolase) is an enzyme with systematic name 2-(acetylamino)-4-O-(2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl)-2-deoxy-D-glucopyranose acetylhydrolase.^{[1] [2] [3] [4]} This enzyme catalyses the following chemical reaction

2-(acetylamino)-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-2-deoxy-beta-D-glucopyranose + H₂O

\rightleftharpoons 2-(acetylamino)-4-O-(2-amino-2-deoxy-beta-D-glucopyranosyl)-2-deoxy-beta-D-glucopyranose + acetate

Chitin oligosaccharide deacetylase is present in Vibrio strains.

Notes and References

1. Kadokura K, Rokutani A, Yamamoto M, Ikegami T, Sugita H, Itoi S, Hakamata W, Oku T, Nishio T . Purification and characterization of Vibrio parahaemolyticus extracellular chitinase and chitin oligosaccharide deacetylase involved in the production of heterodisaccharide from chitin . Applied Microbiology and Biotechnology . 75 . 2 . 357–65 . May 2007 . 17334758 . 10.1007/s00253-006-0831-6 .
2. Hirano T, Kadokura K, Ikegami T, Shigeta Y, Kumaki Y, Hakamata W, Oku T, Nishio T . Heterodisaccharide 4-O-(N-acetyl-beta-D-glucosaminyl)-D-glucosamine is a specific inducer of chitinolytic enzyme production in Vibrios harboring chitin oligosaccharide deacetylase genes . Glycobiology . 19 . 9 . 1046–53 . September 2009 . 19553519 . 10.1093/glycob/cwp088 . free .
3. Purification and properties of two deacetylases produced by Vibrio alginolyticus H-8 . Ohishi K, Yamagishi M, Ohta T, Motosugi M, Izumida H, Sano H, Adachi K, Miwa T . Biosci. Biotechnol. Biochem. . 1997 . 61 . 1113–1117 . 10.1271/bbb.61.1113.
4. Ohishi K, Murase K, Ohta T, Etoh H . Cloning and sequencing of the deacetylase gene from Vibrio alginolyticus H-8 . Journal of Bioscience and Bioengineering . 90 . 5 . 561–3 . 2000 . 16232910 . 10.1263/jbb.90.561 .