| Body: | the Armed Forces of Uzbekistan |
| Native Name: | O'zbekiston Mudofaa vazirligining Bosh shtabi boshlig'i |
| Flag: | Flag of the Ministry of defence of Uzbekistan.jpg |
| Flagsize: | 125px |
| Flagcaption: | Flag of the Ministry of Defence |
| Insigniasize: | 110px |
| Insigniacaption: | Seal of the Joint Staff |
| Incumbent: | Major General Shukhrat Kholmukhamedov |
| Incumbentsince: | 23 March 2021 |
| Department: | Ministry of Defence |
| Member Of: | General Staff of Armed Forces of Uzbekistan National Security Council |
| Reports To: | Minister of Defense |
| Seat: | Tashkent |
| Nominator: | Prime Minister of Uzbekistan |
| Appointer: | President of Uzbekistan |
| Constituting Instrument: | Constitution of Uzbekistan |
| Precursor: | Chief of the General Staff of the Soviet Armed Forces |
| Formation: | September 2000 |
| Deputy: | Deputy Chief of the General Staff |
| Post: | Chief of the General Staff |
The Chief of the General Staff of the Ministry of Defense of Uzbekistan is the highest-ranking military officer of in the Armed Forces of the Republic of Uzbekistan, who is responsible for maintaining the operational command of the military and control over three of the five service branches (Uzbek Ground Forces, Uzbek Air Forces and the Uzbek Naval Forces). Unlike the Minister of Defence, the Chief of the General Staff is required to be a commissioned officer who at least holds the rank of colonel (Polkovnik). Another differentiator between the two positions is that the Chief of the General Staff is not political position while the defense minister can be an active member of the political discourse.
The chief performs the following duties in their role: