| Official Name: | Cheshmeh Talkhu |
| Native Name: | چشمه تلخو |
| Settlement Type: | village |
| Pushpin Map: | Iran |
| Mapsize: | 150px |
| Subdivision Type: | Country |
| Subdivision Type1: | Province |
| Subdivision Name1: | Fars |
| Subdivision Type2: | County |
| Subdivision Name2: | Sepidan |
| Subdivision Type3: | Bakhsh |
| Subdivision Name3: | Hamaijan |
| Subdivision Type4: | Rural District |
| Subdivision Name4: | Hamaijan |
| Population As Of: | 2006 |
| Population Total: | 334 |
| Population Density Km2: | auto |
| Timezone: | IRST |
| Utc Offset: | +3:30 |
| Timezone Dst: | IRDT |
| Utc Offset Dst: | +4:30 |
| Coordinates: | 29.9356°N 52.1017°W |
Cheshmeh Talkhu (Persian: چشمه تلخو, also Romanized as Cheshmeh Talkhū and Cheshmeh-ye Talkhū) is a village in Hamaijan Rural District, Hamaijan District, Sepidan County, Fars Province, Iran. At the 2006 census, its population was 334, in 76 families.