| Official Name: | Cheshmeh Khuni |
| Native Name: | چشمه خوني |
| Settlement Type: | village |
| Pushpin Map: | Iran |
| Mapsize: | 150px |
| Subdivision Type: | Country |
| Subdivision Type1: | Province |
| Subdivision Name1: | Isfahan |
| Subdivision Type2: | County |
| Subdivision Name2: | Semirom |
| Subdivision Type3: | Bakhsh |
| Subdivision Name3: | Central |
| Subdivision Type4: | Rural District |
| Subdivision Name4: | Hana |
| Population As Of: | 2006 |
| Population Total: | 166 |
| Population Density Km2: | auto |
| Timezone: | IRST |
| Utc Offset: | +3:30 |
| Timezone Dst: | IRDT |
| Utc Offset Dst: | +4:30 |
| Coordinates: | 31.1289°N 51.6617°W |
Cheshmeh Khuni (Persian: چشمه خوني, also Romanized as Cheshmeh Khūnī) is a village in Hana Rural District, in the Central District of Semirom County, Isfahan Province, Iran. At the 2006 census, its population was 166, in 40 families.