| Charles du Bois de Vroylande | |
| Birth Name: | Charles Louis du Bois de Vroylande |
| Birth Date: | 1835 1, df=yes |
| Birth Place: | Antwerp, Belgium |
| Death Place: | Antwerp, Belgium |
| Nationality: | Belgian |
| Occupation: | politician |
Jonkheer Charles Louis du Bois de Vroylande (21 January 1835 - 30 December 1888) was a Belgian lawyer and politician. He was governor of the province of Antwerp from 24 March 1887 until 30 December 1888.
Charles du Bois de Vroylande was a member of the Provincial council of Antwerp for the Canton Zandhoven from 3 July 1866 until 23 May 1886, and provincial deputy from 6 July 1876 until 1886. He was a member of the communal council of Halle until 1876 and burgomaster from 1862 until 1876.