| Charles II Otto, Count Palatine of Zweibrücken-Birkenfeld | |
| Noble Family: | House of Wittelsbach |
| Father: | George William, Count Palatine of Zweibrücken-Birkenfeld |
| Mother: | Dorothea of Solms-Sonnenwalde |
| Spouse: | Margaret Hedwig of Hohenlohe-Neuenstein |
| Birth Place: | Birkenfeld |
| Death Place: | Birkenfeld |
| Burial Place: | Meisenheim |
Charles II Otto (German: Karl II. Otto) (5 September 1625 – 30 March 1671) was the Duke of Zweibrücken-Birkenfeld from 1669 until 1671.
Charles Otto was born in Birkenfeld in 1625 as the only son of George William, Count Palatine of Zweibrücken-Birkenfeld. He succeeded his father in 1669. Charles Otto died in Birkenfeld in 1671 and was buried in Meisenheim.
Charles Otto married Margaret Hedwig of Hohenlohe-Neuenstein (1 January 1625 – 24 December 1676), daughter of Count Kraft VII, on 30 November 1616 and had the following children: