Centromere protein E explained

Centromere-associated protein E is a protein that in humans is encoded by the CENPE gene.^{[1] [2]}

Centromere-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centromere-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation.

CENPE is also called Kinesin-7.

Clinical significance

Mutations in CENPE result in autosomal recessive primary microcephaly type 13, which includes skeletal abnormalities and immunodeficiency.^[3]

See also

• CENPF
• CENPJ
• CENPT

Further reading

• Yen TJ . CENP-E is a putative kinetochore motor that accumulates just before mitosis . Nature . 359 . 6395 . 536–9 . 1992 . 1406971 . 10.1038/359536a0 . Li G . Schaar BT . Szilak . Illya . Cleveland . Don W. . 1992Natur.359..536Y . 4306312 .
• Yen TJ . CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase . EMBO J. . 10 . 5 . 1245–54 . 1991 . 2022189 . 10.1002/j.1460-2075.1991.tb08066.x. 452779 . Compton DA . Wise D . Zinkowski . RP . Brinkley . BR . Earnshaw . WC . Cleveland . DW .
• Thrower DA . Mitotic HeLa cells contain a CENP-E-associated minus end-directed microtubule motor . EMBO J. . 14 . 5 . 918–26 . 1995 . 7889940 . 10.1002/j.1460-2075.1995.tb07073.x. 398164 . Jordan MA . Schaar BT . Yen . TJ . Wilson . L .
• Wood KW, Sakowicz R, Goldstein LS, Cleveland DW . CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment . Cell . 91 . 3 . 357–66 . 1997 . 9363944 . 10.1016/S0092-8674(00)80419-5 . 12216144 . free .
• Cooke CA, Schaar B, Yen TJ, Earnshaw WC . Localization of CENP-E in the fibrous corona and outer plate of mammalian kinetochores from prometaphase through anaphase . Chromosoma . 106 . 7 . 446–55 . 1998 . 9391217 . 10.1007/s004120050266 . 18884489 .
• Zecevic M . Active MAP kinase in mitosis: localization at kinetochores and association with the motor protein CENP-E . J. Cell Biol. . 142 . 6 . 1547–58 . 1998 . 9744883 . 10.1083/jcb.142.6.1547 . 2141767 . Catling AD . Eblen ST . Renzi . L . Hittle . JC . Yen . TJ . Gorbsky . GJ . Weber . MJ .
• Chan GK, Schaar BT, Yen TJ . Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1 . J. Cell Biol. . 143 . 1 . 49–63 . 1998 . 9763420 . 10.1083/jcb.143.1.49 . 2132809 .
• Chan GK . Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC . J. Cell Biol. . 146 . 5 . 941–54 . 1999 . 10477750 . 10.1083/jcb.146.5.941 . 2169490 . Jablonski SA . Sudakin V . Hittle . JC . Yen . TJ .
• Ashar HR . Farnesyl transferase inhibitors block the farnesylation of CENP-E and CENP-F and alter the association of CENP-E with the microtubules . J. Biol. Chem. . 275 . 39 . 30451–7 . 2000 . 10852915 . 10.1074/jbc.M003469200 . James L . Gray K . Carr . D . Black . S . Armstrong . L . Bishop . WR . Kirschmeier . P . free .
• Uren AG . Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype . Curr. Biol. . 10 . 21 . 1319–28 . 2001 . 11084331 . 10.1016/S0960-9822(00)00769-7 . Wong L . Pakusch M . Fowler . Kerry J. . Burrows . Francis J. . Vaux . David L. . Choo . K.H.Andy . 18455745 . free . 2000CBio...10.1319U .
• Suzuki Y . Identification and characterization of the potential promoter regions of 1031 kinds of human genes . Genome Res. . 11 . 5 . 677–84 . 2001 . 11337467 . 10.1101/gr.gr-1640r. 311086 . Tsunoda T . Sese J . Taira . H . Mizushima-Sugano . J . Hata . H . Ota . T . Isogai . T . Tanaka . T .
• Liu ST . Human MPS1 kinase is required for mitotic arrest induced by the loss of CENP-E from kinetochores . Mol. Biol. Cell . 14 . 4 . 1638–51 . 2003 . 12686615 . 10.1091/mbc.02-05-0074 . 153128 . Chan GK . Hittle JC . Fujii . G . Lees . E . Yen . TJ .
• Brandenberger R . Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation . Nat. Biotechnol. . 22 . 6 . 707–16 . 2005 . 15146197 . 10.1038/nbt971 . Wei H . Zhang S . Lei . Shirley . Murage . Jaji . Fisk . Gregory J . Li . Yan . Xu . Chunhui . Fang . Rixun . 27764390 .
• Garcia-Saez I, Blot D, Kahn R, Kozielski F . Crystallization and preliminary crystallographic analysis of the motor domain of human kinetochore-associated protein CENP-E using an automated crystallization procedure . Acta Crystallogr. D . 60 . Pt 6 . 1158–60 . 2005 . 15159587 . 10.1107/S0907444904009564 .
• Tanudji M . Gene silencing of CENP-E by small interfering RNA in HeLa cells leads to missegregation of chromosomes after a mitotic delay . Mol. Biol. Cell . 15 . 8 . 3771–81 . 2005 . 15181147 . 10.1091/mbc.E03-07-0482 . 491836 . Shoemaker J . L'Italien L . Russell . L . Chin . G . Schebye . XM .
• Garcia-Saez I, Yen T, Wade RH, Kozielski F . Crystal structure of the motor domain of the human kinetochore protein CENP-E . J. Mol. Biol. . 340 . 5 . 1107–16 . 2004 . 15236970 . 10.1016/j.jmb.2004.05.053 .
• Kurasawa Y . Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation . EMBO J. . 23 . 16 . 3237–48 . 2005 . 15297875 . 10.1038/sj.emboj.7600347 . 514520 . Earnshaw WC . Mochizuki Y . Dohmae . Naoshi . Todokoro . Kazuo .
• Mao Y, Desai A, Cleveland DW . Microtubule capture by CENP-E silences BubR1-dependent mitotic checkpoint signaling . J. Cell Biol. . 170 . 6 . 873–80 . 2005 . 16144904 . 10.1083/jcb.200505040 . 2171436 .
• Nousiainen M . Phosphoproteome analysis of the human mitotic spindle . Proc. Natl. Acad. Sci. U.S.A. . 103 . 14 . 5391–6 . 2006 . 16565220 . 10.1073/pnas.0507066103 . 1459365 . Silljé HH . Sauer G . Nigg . EA . Körner . R . 2006PNAS..103.5391N . free .

Notes and References

1. Testa JR, Zhou JY, Bell DW, Yen TJ . Chromosomal localization of the genes encoding the kinetochore proteins CENPE and CENPF to human chromosomes 4q24→q25 and 1q32→q41, respectively, by fluorescence in situ hybridization . Genomics . 23 . 3 . 691–3 . Mar 1995 . 7851898 . 10.1006/geno.1994.1558 .
2. Web site: Entrez Gene: CENPE centromere protein E, 312kDa.
3. Web site: OMIM Entry - # 616051 - MICROCEPHALY 13, PRIMARY, AUTOSOMAL RECESSIVE; MCPH13. www.omim.org. 2020-01-25.