Centrolene antioquiensis (common name: Antioquia giant glass frog) is a species of frog in the family Centrolenidae. It is endemic to Cordillera Central of Colombia and found in Antioquia, Caldas, and Tolima Departments at elevations of 1850m-2450mm (6,070feet-8,040feetm) above sea level.
Centrolene antioquiensis inhabits vegetation alongside streams in sub-Andean forests. Water pollution from agriculture, and more locally deforestation, threatens this species.