Centrin 3 Explained

Centrin-3 is a protein that in humans is encoded by the CETN3 gene.^{[1] [2]} It belongs to the centrin family of proteins.

The protein encoded by this gene contains four EF-hand calcium binding domains, and is a member of the centrin protein family. Centrins are evolutionarily conserved proteins similar to the CDC31 protein of S. cerevisiae. Yeast CDC31 is located at the centrosome of interphase and mitotic cells, where it plays a fundamental role in centrosome duplication and separation. Multiple forms of the proteins similar to the yeast centrin have been identified in human and other mammalian cells, some of which have been shown to be associated with centrosome fractions. This protein appears to be one of the most abundant centrins associated with centrosome, which suggests a similar function to its yeast counterpart.

Further reading

• Salisbury JL . Centrosomes: Sfi1p and centrin unravel a structural riddle . Curr. Biol. . 14 . 1 . R27–9 . 2004 . 14711432 . 10.1016/j.cub.2003.12.019 . 16717091 . free . 2004CBio...14..R27S .
• Wiech H, Geier BM, Paschke T . Characterization of green alga, yeast, and human centrins. Specific subdomain features determine functional diversity . J. Biol. Chem. . 271 . 37 . 22453–61 . 1996 . 8798410 . 10.1074/jbc.271.37.22453 . etal. free .
• Paoletti A, Moudjou M, Paintrand M . Most of centrin in animal cells is not centrosome-associated and centrosomal centrin is confined to the distal lumen of centrioles . J. Cell Sci. . 109 . 13. 3089–102 . 1997 . 10.1242/jcs.109.13.3089 . 9004043 . etal.
• LeDizet M, Beck JC, Finkbeiner WE . Differential regulation of centrin genes during ciliogenesis in human tracheal epithelial cells . Am. J. Physiol. . 275 . 6 Pt 1 . L1145–56 . 1999 . 9843852 . 10.1152/ajplung.1998.275.6.L1145.
• Laoukili J, Perret E, Middendorp S . Differential expression and cellular distribution of centrin isoforms during human ciliated cell differentiation in vitro . J. Cell Sci. . 113 . 8. 1355–64 . 2000 . 10.1242/jcs.113.8.1355 . 10725219 . etal.
• Lutz W, Lingle WL, McCormick D . Phosphorylation of centrin during the cell cycle and its role in centriole separation preceding centrosome duplication . J. Biol. Chem. . 276 . 23 . 20774–80 . 2001 . 11279195 . 10.1074/jbc.M101324200 . etal. free .
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Schiebel E, Bornens M . In search of a function for centrins . Trends Cell Biol. . 5 . 5 . 197–201 . 2004 . 14731449 . 10.1016/S0962-8924(00)88999-0 .
• Gerhard DS, Wagner L, Feingold EA . The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Cox JA, Tirone F, Durussel I . Calcium and magnesium binding to human centrin 3 and interaction with target peptides . Biochemistry . 44 . 3 . 840–50 . 2005 . 15654740 . 10.1021/bi048294e . etal.
• Poole E, Strappe P, Mok HP . HIV-1 Gag-RNA interaction occurs at a perinuclear/centrosomal site; analysis by confocal microscopy and FRET . Traffic . 6 . 9 . 741–55 . 2005 . 16101678 . 10.1111/j.1600-0854.2005.00312.x . 36574274 . etal.
• Stelzl U, Worm U, Lalowski M . A human protein-protein interaction network: a resource for annotating the proteome . Cell . 122 . 6 . 957–68 . 2005 . 16169070 . 10.1016/j.cell.2005.08.029 . etal. 11858/00-001M-0000-0010-8592-0 . 8235923 . free .

Notes and References

1. Middendorp S, Paoletti A, Schiebel E, Bornens M . Identification of a new mammalian centrin gene, more closely related to Saccharomyces cerevisiae CDC31 gene . Proc Natl Acad Sci U S A . 94 . 17 . 9141–6 . Sep 1997 . 9256449 . 23077 . 10.1073/pnas.94.17.9141 . 1997PNAS...94.9141M . free .
2. Web site: Entrez Gene: CETN3 centrin, EF-hand protein, 3 (CDC31 homolog, yeast).