Centrin 2 Explained

Centrin-2 is a protein that in humans is encoded by the CETN2 gene.^{[1] [2] [3]} It belongs to the centrin family of proteins.

Centrin-2 belongs to a family of calcium-binding proteins and is a structural component of the centrosome. The high level of conservation from algae to humans and its association with the centrosome suggested that centrin-2 plays a fundamental role in the structure and function of the microtubule-organizing center, possibly required for the proper duplication and segregation of the centrosome.

Interactions

CETN2 has been shown to interact with XPC^[4] and SFI1.^[5]

Further reading

• Lee VD, Huang B . Molecular cloning and centrosomal localization of human caltractin. . Proc. Natl. Acad. Sci. U.S.A. . 90 . 23 . 11039–43 . 1994 . 8248209 . 10.1073/pnas.90.23.11039 . 47917 . 1993PNAS...9011039L . free .
• Wolfrum U, Salisbury JL . 8459226 . Expression of centrin isoforms in the mammalian retina. . Exp. Cell Res. . 242 . 1 . 10–7 . 1998 . 9665797 . 10.1006/excr.1998.4038 .
• Durussel I, Blouquit Y, Middendorp S . Cation- and peptide-binding properties of human centrin 2. . FEBS Lett. . 472 . 2–3 . 208–12 . 2000 . 10788612 . 10.1016/S0014-5793(00)01452-6 . etal. free .
• Mallon AM, Platzer M, Bate R . Comparative genome sequence analysis of the Bpa/Str region in mouse and Man. . Genome Res. . 10 . 6 . 758–75 . 2000 . 10854409 . 10.1101/gr.10.6.758 . 310879 . etal.
• Hartley JL, Temple GF, Brasch MA . DNA cloning using in vitro site-specific recombination. . Genome Res. . 10 . 11 . 1788–95 . 2001 . 11076863 . 10.1101/gr.143000 . 310948 .
• Simpson JC, Wellenreuther R, Poustka A . Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. . EMBO Rep. . 1 . 3 . 287–92 . 2001 . 11256614 . 10.1093/embo-reports/kvd058 . 1083732 . etal.
• Araki M, Masutani C, Takemura M . Centrosome protein centrin 2/caltractin 1 is part of the xeroderma pigmentosum group C complex that initiates global genome nucleotide excision repair. . J. Biol. Chem. . 276 . 22 . 18665–72 . 2001 . 11279143 . 10.1074/jbc.M100855200 . etal. free .
• Salisbury JL, Suino KM, Busby R, Springett M . Centrin-2 is required for centriole duplication in mammalian cells. . Curr. Biol. . 12 . 15 . 1287–92 . 2003 . 12176356 . 10.1016/S0960-9822(02)01019-9 . free .
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . etal. 2002PNAS...9916899M . free .
• Matei E, Miron S, Blouquit Y . C-terminal half of human centrin 2 behaves like a regulatory EF-hand domain. . Biochemistry . 42 . 6 . 1439–50 . 2003 . 12578356 . 10.1021/bi0269714 . etal.
• Gavet O, Alvarez C, Gaspar P, Bornens M . Centrin4p, a novel mammalian centrin specifically expressed in ciliated cells. . Mol. Biol. Cell . 14 . 5 . 1818–34 . 2004 . 12802058 . 10.1091/mbc.E02-11-0709 . 165080 .
• Popescu A, Miron S, Blouquit Y . Xeroderma pigmentosum group C protein possesses a high affinity binding site to human centrin 2 and calmodulin. . J. Biol. Chem. . 278 . 41 . 40252–61 . 2003 . 12890685 . 10.1074/jbc.M302546200 . etal. free .
• Kilmartin JV . Sfi1p has conserved centrin-binding sites and an essential function in budding yeast spindle pole body duplication. . J. Cell Biol. . 162 . 7 . 1211–21 . 2003 . 14504268 . 10.1083/jcb.200307064 . 2173958 .
• Chang F, Re F, Sebastian S . HIV-1 Vpr induces defects in mitosis, cytokinesis, nuclear structure, and centrosomes. . Mol. Biol. Cell . 15 . 4 . 1793–801 . 2004 . 14767062 . 10.1091/mbc.E03-09-0691 . 379276 . etal.
• Tourbez M, Firanescu C, Yang A . Calcium-dependent self-assembly of human centrin 2. . J. Biol. Chem. . 279 . 46 . 47672–80 . 2005 . 15356003 . 10.1074/jbc.M404996200 . etal. free .
• Gerhard DS, Wagner L, Feingold EA . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Wiemann S, Arlt D, Huber W . From ORFeome to biology: a functional genomics pipeline. . Genome Res. . 14 . 10B . 2136–44 . 2004 . 15489336 . 10.1101/gr.2576704 . 528930 . etal.
• Nishi R, Okuda Y, Watanabe E . Centrin 2 stimulates nucleotide excision repair by interacting with xeroderma pigmentosum group C protein. . Mol. Cell. Biol. . 25 . 13 . 5664–74 . 2005 . 15964821 . 10.1128/MCB.25.13.5664-5674.2005 . 1156980 . etal.

Notes and References

1. Tanaka T, Okui K, Nakamura Y . Assignment of the human caltractin gene (CALT) to Xq28 by fluorescence in situ hybridization . Genomics . 24 . 3 . 609–10 . May 1995 . 7713520 .
2. Chatterjee A, Tanaka T, Parrish JE, Herman GE . Refined mapping of caltractin in human Xq28 and in the homologous region of the mouse X chromosome places the gene within the bare patches (Bpa) and striated (Str) critical regions . Mamm Genome . 6 . 11 . 802–4 . Apr 1996 . 8597638 . 10.1007/BF00539008 . 19420279 .
3. Web site: Entrez Gene: CETN2 centrin, EF-hand protein, 2.
4. Araki . M . Masutani C. Takemura M. Uchida A. Sugasawa K. Kondoh J. Ohkuma Y. Hanaoka F . Jun 2001 . Centrosome protein centrin 2/caltractin 1 is part of the xeroderma pigmentosum group C complex that initiates global genome nucleotide excision repair . J. Biol. Chem. . 276 . 22 . 18665–72 . United States. 0021-9258. 11279143 . 10.1074/jbc.M100855200 . free .
5. Martinez-Sanz. J. Kateb. F. Assairi. L. Blouquit. Y. Bodenhausen. G. Abergel. D. Mouawad. L. Craescu. CT. Structure, dynamics and thermodynamics of the human centrin 2/hSfi1 complex.. Journal of Molecular Biology. Jan 8, 2010. 395. 1. 191–204. 19857500. 10.1016/j.jmb.2009.10.041.