| Caloosahatchee National Wildlife Refuge | |
| Iucn Category: | V |
| Map: | USA |
| Relief: | 1 |
| Location: | North Fort Myers, Florida, United States |
| Nearest City: | North Fort Myers, Florida |
| Coordinates: | 26.6983°N -81.7972°W |
| Area: | 40acres |
| Established: | January 1, 1921 |
| Governing Body: | US Fish & Wildlife Service |
| Website: | Caloosahatchee National Wildlife Refuge |
The Caloosahatchee National Wildlife Refuge is part of the United States National Wildlife Refuge System, located on the Caloosahatchee River, beneath the I-75 Caloosahatchee Bridge, within the city of North Fort Myers. The 40acres refuge was established on January 1, 1921. It is administered as part of the J. N. "Ding" Darling National Wildlife Refuge Complex.