Calf-intestinal alkaline phosphatase explained

Calf-intestinal alkaline phosphatase
Width:	200
Organism:	Bos taurus
Taxid:	9913
Symbol:	ALPI
Uniprot:	P19111

Calf-intestinal alkaline phosphatase (CIAP/CIP) is a type of alkaline phosphatase that catalyzes the removal of phosphate groups from the 5' end of DNA strands and phosphomonoesters from RNA. This enzyme is frequently used in DNA sub-cloning, as DNA fragments that lack the 5' phosphate groups cannot ligate. This prevents recircularization of the linearized DNA vector and improves the yield of the vector containing the appropriate insert.

Applications

Calf-intestinal alkaline phosphatase can serve as an effective tool for removing uranium from groundwater and soil that can pose major health risks.^[1] Furthermore, the toxicity of lipopolysaccharide (LPS) was mitigated by calf-intestinal alkaline phosphatase in mice and piglets, which indicates that it could be a promising new therapeutic agent for treating diseases associated with LPS.^[2]

Notes and References

1. Chaudhuri G, Dey P, Dalal D, Venu-Babu P, Thilagaraj WR . July 2013 . A Novel Approach to Precipitation of Heavy Metals from Industrial Effluents and Single-Ion Solutions Using Bacterial Alkaline Phosphatase . Water, Air, & Soil Pollution . en . 224 . 7 . 1625 . 10.1007/s11270-013-1625-y . 2013WASP..224.1625C . 97367168 . 0049-6979.
2. Beumer C, Wulferink M, Raaben W, Fiechter D, Brands R, Seinen W . Calf intestinal alkaline phosphatase, a novel therapeutic drug for lipopolysaccharide (LPS)-mediated diseases, attenuates LPS toxicity in mice and piglets . The Journal of Pharmacology and Experimental Therapeutics . 307 . 2 . 737–744 . November 2003 . 12970380 . 10.1124/jpet.103.056606 . 15049304 .