CYP12 family explained

Cytochrome P450, family 12, also known as CYP12, is a cytochrome P450 family found in insect genome belongs to Mitochondrial clan CYPs, which is located in the inner membrane of mitochondria(IMM).^{[1] [2]} The first gene identified in this family is the CYP12A1 from the Musca domestica (house fly), which is involved in insecticide resistance.^[3] CYP12A1 protein localization in mitochondria by immunohistochemistry and absolute dependence on mitochondrial electron donors adrenodoxin reductase and adrenodoxin.^[3]

Rabbit gene CYP8B1 was named CYP12 at the beginning of its discovery, because it hydroxylated its sterol substrate on the 12 position.^[4] However, CYP12 is a family of insect P450s found in mitochondria, so this gene was renamed to CYP8B1.^[5]

Notes and References

1. Nelson . DR . Metazoan cytochrome P450 evolution. . Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology . November 1998 . 121 . 1–3 . 15–22 . 10.1016/s0742-8413(98)10027-0 . 9972448.
2. Tijet . N . Helvig . C . Feyereisen . R . The cytochrome P450 gene superfamily in Drosophila melanogaster: annotation, intron-exon organization and phylogeny. . Gene . 10 January 2001 . 262 . 1–2 . 189–98 . 10.1016/s0378-1119(00)00533-3 . 11179683.
3. Guzov . VM . Unnithan . GC . Chernogolov . AA . Feyereisen . R . CYP12A1, a mitochondrial cytochrome P450 from the house fly. . Archives of Biochemistry and Biophysics . 15 November 1998 . 359 . 2 . 231–40 . 10.1006/abbi.1998.0901 . 9808765.
4. Eggertsen . G . Olin . M . Andersson . U . Ishida . H . Kubota . S . Hellman . U . Okuda . KI . Björkhem . I . Molecular cloning and expression of rabbit sterol 12alpha-hydroxylase. . The Journal of Biological Chemistry . 13 December 1996 . 271 . 50 . 32269–75 . 10.1074/jbc.271.50.32269 . 8943286. 84628848 . free .
5. Book: Nelson . DR . Cytochrome P450 Protocols . Cytochrome P450 Nomenclature, 2004 . Methods in Molecular Biology . 2006 . 320 . 1–10 . 10.1385/1-59259-998-2:1 . 16719369. 1-59259-998-2 .