CTDP1 explained

RNA polymerase II subunit A C-terminal domain phosphatase is an enzyme that in humans is encoded by the CTDP1 gene.^{[1] [2] [3]}

This gene encodes a protein which interacts with the carboxy-terminus of transcription initiation factor TFIIF, a transcription factor which regulates elongation as well as initiation by RNA polymerase II. The protein may also represent a component of an RNA polymerase II holoenzyme complex. Alternative splicing of this gene results in two transcript variants encoding 2 different isoforms.^[4]

Interactions

CTDP1 has been shown to interact with WD repeat-containing protein 77,^[5] GTF2F1 and POLR2A.^[6]

Further reading

• Scully R, Anderson SF, Chao DM . BRCA1 is a component of the RNA polymerase II holoenzyme. . Proc. Natl. Acad. Sci. U.S.A. . 94 . 11 . 5605–10 . 1997 . 9159119 . 10.1073/pnas.94.11.5605 . 20825 . etal. 1997PNAS...94.5605S. free .
• Marshall NF, Dahmus GK, Dahmus ME . Regulation of carboxyl-terminal domain phosphatase by HIV-1 tat protein. . J. Biol. Chem. . 273 . 48 . 31726–30 . 1998 . 9822634 . 10.1074/jbc.273.48.31726 . free .
• Cho H, Kim TK, Mancebo H . A protein phosphatase functions to recycle RNA polymerase II. . Genes Dev. . 13 . 12 . 1540–52 . 1999 . 10385623 . 10.1101/gad.13.12.1540 . 316795 . etal.
• Angelicheva D, Turnev I, Dye D . Congenital cataracts facial dysmorphism neuropathy (CCFDN) syndrome: a novel developmental disorder in Gypsies maps to 18qter. . Eur. J. Hum. Genet. . 7 . 5 . 560–6 . 1999 . 10439962 . 10.1038/sj.ejhg.5200319 . etal. free .
• Marshall NF, Dahmus ME . C-terminal domain phosphatase sensitivity of RNA polymerase II in early elongation complexes on the HIV-1 and adenovirus 2 major late templates. . J. Biol. Chem. . 275 . 42 . 32430–7 . 2000 . 10938286 . 10.1074/jbc.M005898200 . free .
• Licciardo P, Napolitano G, Majello B, Lania L . Inhibition of Tat transactivation by the RNA polymerase II CTD-phosphatase FCP1. . AIDS . 15 . 3 . 301–7 . 2001 . 11273209 . 10.1097/00002030-200102160-00002 . 33054695 . free .
• Bharucha DC, Zhou M, Nekhai S . A protein phosphatase from human T cells augments tat transactivation of the human immunodeficiency virus type 1 long-terminal repeat. . Virology . 296 . 1 . 6–16 . 2002 . 12036313 . 10.1006/viro.2002.1438 . etal. free .
• Washington K, Ammosova T, Beullens M . Protein phosphatase-1 dephosphorylates the C-terminal domain of RNA polymerase-II. . J. Biol. Chem. . 277 . 43 . 40442–8 . 2002 . 12185079 . 10.1074/jbc.M205687200 . etal. free .
• Mandal SS, Cho H, Kim S . FCP1, a phosphatase specific for the heptapeptide repeat of the largest subunit of RNA polymerase II, stimulates transcription elongation. . Mol. Cell. Biol. . 22 . 21 . 7543–52 . 2002 . 12370301 . 10.1128/MCB.22.21.7543-7552.2002 . 135672 . etal.
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . etal. 2002PNAS...9916899M . free .
• Licciardo P, Amente S, Ruggiero L . The FCP1 phosphatase interacts with RNA polymerase II and with MEP50 a component of the methylosome complex involved in the assembly of snRNP. . Nucleic Acids Res. . 31 . 3 . 999–1005 . 2003 . 12560496 . 10.1093/nar/gkg197 . 149217 . etal.
• Nguyen BD, Chen HT, Kobor MS . Solution structure of the carboxyl-terminal domain of RAP74 and NMR characterization of the FCP1-binding sites of RAP74 and human TFIIB. . Biochemistry . 42 . 6 . 1460–9 . 2003 . 12578358 . 10.1021/bi0265473 . etal.
• Friedl EM, Lane WS, Erdjument-Bromage H . The C-terminal domain phosphatase and transcription elongation activities of FCP1 are regulated by phosphorylation. . Proc. Natl. Acad. Sci. U.S.A. . 100 . 5 . 2328–33 . 2003 . 12591939 . 10.1073/pnas.2628049100 . 151340 . etal. 2003PNAS..100.2328F . free .
• Kamada K, Roeder RG, Burley SK . Molecular mechanism of recruitment of TFIIF- associating RNA polymerase C-terminal domain phosphatase (FCP1) by transcription factor IIF. . Proc. Natl. Acad. Sci. U.S.A. . 100 . 5 . 2296–9 . 2003 . 12591941 . 10.1073/pnas.262798199 . 151334 . 2003PNAS..100.2296K . free .
• Nguyen BD, Abbott KL, Potempa K . NMR structure of a complex containing the TFIIF subunit RAP74 and the RNA polymerase II carboxyl-terminal domain phosphatase FCP1. . Proc. Natl. Acad. Sci. U.S.A. . 100 . 10 . 5688–93 . 2003 . 12732728 . 10.1073/pnas.1031524100 . 156262 . etal. 2003PNAS..100.5688N . free .
• Varon R, Gooding R, Steglich C . Partial deficiency of the C-terminal-domain phosphatase of RNA polymerase II is associated with congenital cataracts facial dysmorphism neuropathy syndrome. . Nat. Genet. . 35 . 2 . 185–9 . 2003 . 14517542 . 10.1038/ng1243 . 22461437 . etal.
• Yu X, Chini CC, He M . The BRCT domain is a phospho-protein binding domain. . Science . 302 . 5645 . 639–42 . 2003 . 14576433 . 10.1126/science.1088753 . etal. 2003Sci...302..639Y . 29407635 .
• Beausoleil SA, Jedrychowski M, Schwartz D . Large-scale characterization of HeLa cell nuclear phosphoproteins. . Proc. Natl. Acad. Sci. U.S.A. . 101 . 33 . 12130–5 . 2004 . 15302935 . 10.1073/pnas.0404720101 . 514446 . etal. 2004PNAS..10112130B . free .

Notes and References

1. Archambault J, Chambers RS, Kobor MS, Ho Y, Cartier M, Bolotin D, Andrews B, Kane CM, Greenblatt J . An essential component of a C-terminal domain phosphatase that interacts with transcription factor IIF in Saccharomyces cerevisiae . Proc Natl Acad Sci U S A . 94 . 26 . 14300–5 . Feb 1998 . 9405607 . 24951 . 10.1073/pnas.94.26.14300 . 1997PNAS...9414300A . free .
2. Archambault J, Pan G, Dahmus GK, Cartier M, Marshall N, Zhang S, Dahmus ME, Greenblatt J . FCP1, the RAP74-interacting subunit of a human protein phosphatase that dephosphorylates the carboxyl-terminal domain of RNA polymerase IIO . J Biol Chem . 273 . 42 . 27593–601 . Nov 1998 . 9765293 . 10.1074/jbc.273.42.27593 . free .
3. Web site: Entrez Gene: CTDP1 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1.
4. Web site: Entrez Gene: CTDP1 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1.
5. Licciardo . Paolo . Amente Stefano . Ruggiero Luca . Monti Maria . Pucci Piero . Lania Luigi . Majello Barbara . Feb 2003 . The FCP1 phosphatase interacts with RNA polymerase II and with MEP50 a component of the methylosome complex involved in the assembly of snRNP . Nucleic Acids Res. . 31 . 3 . 999–1005 . England. 12560496 . 10.1093/nar/gkg197 . 149217 .
6. Scully . R . Anderson S F . Chao D M . Wei W . Ye L . Young R A . Livingston D M . Parvin J D . May 1997 . BRCA1 is a component of the RNA polymerase II holoenzyme . . 94 . 11 . 5605–10 . UNITED STATES. 0027-8424. 9159119 . 1997PNAS...94.5605S . 10.1073/pnas.94.11.5605 . 20825 . free .