CSTF3 explained

Cleavage stimulation factor 77 kDa subunit is a protein that in humans is encoded by the CSTF3 gene.^{[1] [2]}

The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms.

Interactions

CSTF3 has been shown to interact with CSTF2.^[3]

Further reading

• Takagaki Y, Manley JL . A human polyadenylation factor is a G protein beta-subunit homologue. . J. Biol. Chem. . 267 . 33 . 23471–4 . 1992 . 10.1016/S0021-9258(18)35861-7 . 1358884 . free .
• Takagaki Y, MacDonald CC, Shenk T, Manley JL . The human 64-kDa polyadenylylation factor contains a ribonucleoprotein-type RNA binding domain and unusual auxiliary motifs. . Proc. Natl. Acad. Sci. U.S.A. . 89 . 4 . 1403–7 . 1992 . 1741396 . 10.1073/pnas.89.4.1403. 48459 . 1992PNAS...89.1403T . free .
• Murthy KG, Manley JL . The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation. . Genes Dev. . 9 . 21 . 2672–83 . 1995 . 7590244 . 10.1101/gad.9.21.2672 . free .
• Takagaki Y, Manley JL . Complex protein interactions within the human polyadenylation machinery identify a novel component. . Mol. Cell. Biol. . 20 . 5 . 1515–25 . 2000 . 10669729 . 10.1128/MCB.20.5.1515-1525.2000. 85326 .
• Emili A, Schieltz DM, Yates JR, Hartwell LH . Dynamic interaction of DNA damage checkpoint protein Rad53 with chromatin assembly factor Asf1. . Mol. Cell . 7 . 1 . 13–20 . 2001 . 11172707 . 10.1016/S1097-2765(01)00150-2 . free .
• Benoit B, Juge F, Iral F . Chimeric human CstF-77/Drosophila Suppressor of forked proteins rescue suppressor of forked mutant lethality and mRNA 3' end processing in Drosophila. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 16 . 10593–8 . 2002 . 12149458 . 10.1073/pnas.162191899 . 124984 . etal. 2002PNAS...9910593B . free .
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . etal. 2002PNAS...9916899M . free .
• Kaufmann I, Martin G, Friedlein A . Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase. . EMBO J. . 23 . 3 . 616–26 . 2005 . 14749727 . 10.1038/sj.emboj.7600070 . 1271804 . etal.
• Gerhard DS, Wagner L, Feingold EA . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Kim J, Bhinge AA, Morgan XC, Iyer VR . Mapping DNA-protein interactions in large genomes by sequence tag analysis of genomic enrichment. . Nat. Methods . 2 . 1 . 47–53 . 2005 . 15782160 . 10.1038/nmeth726 . 6135437 .
• Rual JF, Venkatesan K, Hao T . Towards a proteome-scale map of the human protein-protein interaction network. . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . etal. 2005Natur.437.1173R . 4427026 .
• Taylor TD, Noguchi H, Totoki Y . Human chromosome 11 DNA sequence and analysis including novel gene identification. . Nature . 440 . 7083 . 497–500 . 2006 . 16554811 . 10.1038/nature04632 . etal. 2006Natur.440..497T . free .
• Olsen JV, Blagoev B, Gnad F . Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. . Cell . 127 . 3 . 635–48 . 2006 . 17081983 . 10.1016/j.cell.2006.09.026 . 7827573 . etal. free .
• Ewing RM, Chu P, Elisma F . Large-scale mapping of human protein-protein interactions by mass spectrometry. . Mol. Syst. Biol. . 3 . 1. 89 . 2007 . 17353931 . 10.1038/msb4100134 . 1847948 . etal.

Notes and References

1. Takagaki Y, Manley JL . A polyadenylation factor subunit is the human homologue of the Drosophila suppressor of forked protein . Nature . 372 . 6505 . 471–4 . December 1994 . 7984242 . 10.1038/372471a0 . 1994Natur.372..471T . 4329672 .
2. Web site: Entrez Gene: CSTF3 cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa.
3. 10.1128/MCB.20.5.1515-1525.2000 . Takagaki . Y . Manley J L . March 2000 . Complex protein interactions within the human polyadenylation machinery identify a novel component . Mol. Cell. Biol. . 20 . 5 . 1515–25 . 0270-7306. 10669729 . 85326 .