CSTF1 explained

Cleavage stimulation factor 50 kDa subunit is a protein that in humans is encoded by the CSTF1 gene.^{[1] [2]}

This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene.^[3]

Interactions

CSTF1 has been shown to interact with BARD1.^[4]

Further reading

• Takagaki Y, MacDonald CC, Shenk T, Manley JL . The human 64-kDa polyadenylylation factor contains a ribonucleoprotein-type RNA binding domain and unusual auxiliary motifs . Proceedings of the National Academy of Sciences of the United States of America . 89 . 4 . 1403–7 . February 1992 . 1741396 . 48459 . 10.1073/pnas.89.4.1403 . 1992PNAS...89.1403T . free .
• Murthy KG, Manley JL . The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation . Genes & Development . 9 . 21 . 2672–83 . November 1995 . 7590244 . 10.1101/gad.9.21.2672 . free .
• Takagaki Y, Manley JL . A polyadenylation factor subunit is the human homologue of the Drosophila suppressor of forked protein . Nature . 372 . 6505 . 471–4 . December 1994 . 7984242 . 10.1038/372471a0 . 1994Natur.372..471T . 4329672 .
• Rüegsegger U, Beyer K, Keller W . Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors . The Journal of Biological Chemistry . 271 . 11 . 6107–13 . March 1996 . 8626397 . 10.1074/jbc.271.11.6107 . free .
• Kleiman FE, Manley JL . Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50 . Science . 285 . 5433 . 1576–9 . September 1999 . 10477523 . 10.1126/science.285.5433.1576 .
• Takagaki Y, Manley JL . Complex protein interactions within the human polyadenylation machinery identify a novel component . Molecular and Cellular Biology . 20 . 5 . 1515–25 . March 2000 . 10669729 . 85326 . 10.1128/MCB.20.5.1515-1525.2000 .
• Fong N, Bentley DL . Capping, splicing, and 3' processing are independently stimulated by RNA polymerase II: different functions for different segments of the CTD . Genes & Development . 15 . 14 . 1783–95 . July 2001 . 11459828 . 312735 . 10.1101/gad.889101 .
• Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI . Directed proteomic analysis of the human nucleolus . Current Biology . 12 . 1 . 1–11 . January 2002 . 11790298 . 10.1016/S0960-9822(01)00650-9 . 14132033 . free . 2002CBio...12....1A .
• Rush J, Moritz A, Lee KA, Guo A, Goss VL, Spek EJ, Zhang H, Zha XM, Polakiewicz RD, Comb MJ . Immunoaffinity profiling of tyrosine phosphorylation in cancer cells . Nature Biotechnology . 23 . 1 . 94–101 . January 2005 . 15592455 . 10.1038/nbt1046 . 7200157 .
• Tsuzuki M, Wu W, Nishikawa H, Hayami R, Oyake D, Yabuki Y, Fukuda M, Ohta T . A truncated splice variant of human BARD1 that lacks the RING finger and ankyrin repeats . Cancer Letters . 233 . 1 . 108–16 . February 2006 . 15878232 . 10.1016/j.canlet.2005.03.012 .

Notes and References

1. Takagaki Y, Manley JL . A human polyadenylation factor is a G protein beta-subunit homologue . The Journal of Biological Chemistry . 267 . 33 . 23471–4 . November 1992 . 10.1016/S0021-9258(18)35861-7 . 1358884 . free .
2. Kleiman FE, Manley JL . The BARD1-CstF-50 interaction links mRNA 3' end formation to DNA damage and tumor suppression . Cell . 104 . 5 . 743–53 . March 2001 . 11257228 . 10.1016/S0092-8674(01)00270-7 . 15022237 . free .
3. Web site: Entrez Gene: CSTF1 cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa.
4. Kleiman FE, Manley JL . Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50 . Science . 285 . 5433 . 1576–9 . September 1999 . 10477523 . 10.1126/science.285.5433.1576 .