CSPD (molecule) explained

CSPD ([3-(1-chloro-3'-methoxyspiro[adamantane-4,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate) is a chemical substance with formula C₁₈H₂₂ClO₇P. It is a component of enhanced chemiluminescence enzyme-linked immunosorbent assay (ELISA) kits, used for the detection of minute amounts of various substances such as proteins.^[1]

Properties

The molecule CSPD has the following functional groups in the structure: phosphate group, phenyl group, spiro group, methyl ether group, and chlorine group. The ones worth noting are the ones above. None of these groups carry a charge. If there was a charge this would have had a change in the compound's pH, 3D structure, mass and bond angles.^[2]

The toxin CSPD effect persister cell formation using MqsR (MqsR, a crucial regulator for quorum sensing and biofilm formation, is a GCU-specific mRNA interferase in Escherichia coli^[3]) and persister cells are cells that avoid stress and are characterized by reduced metabolism and other factors.^[4]

Notes and References

1. 10.1016/0009-9120(93)90108-I. Ultrasensitive immunoassay techniques. Clinical Biochemistry. 26. 5. 325–331. 1993. Kricka. Larry J. 8299202. free.
2. Web site: PubChem. [3-(1-Chloro-3'-methoxyspiro[adamantane-4,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate]. 2021-11-18. pubchem.ncbi.nlm.nih.gov. en.
3. Web site: mqsR - mRNA interferase toxin MqsR - Escherichia coli (strain K12) - mqsR gene & protein. 2021-11-19. www.uniprot.org. en.
4. Kim. Younghoon. Wood. Thomas K.. 2010-01-01. Toxins Hha and CspD and small RNA regulator Hfq are involved in persister cell formation through MqsR in Escherichia coli. Biochemical and Biophysical Research Communications. en. 391. 1. 209–213. 10.1016/j.bbrc.2009.11.033. 19909729. 2812665. 0006-291X.