CPSF2 explained

Cleavage and polyadenylation specificity factor subunit 2 is a protein that in humans is encoded by the CPSF2 gene.^{[1] [2] [3]} This protein is a subunit of the cleavage and polyadenylation specificity factor (CPSF) complex which plays a key role in pre-mRNA 3' end processing and polyadenylation.^[4] The CPSF2 protein connects the two subunits of the complex, mCF and mPSF. Its structure contributes both to the stability of the subunits interaction and to the flexibility of the complex necessary for function.^[5] This protein has been identified as an essential subunit of the complex as certain mutations in the region inhibit CPSF complex formation.

Further reading

• Murthy KG, Manley JL . The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation. . Genes Dev. . 9 . 21 . 2672–83 . 1995 . 7590244 . 10.1101/gad.9.21.2672 . free .
• Thuresson AC, Aström J, Aström A . Multiple forms of poly(A) polymerases in human cells. . Proc. Natl. Acad. Sci. U.S.A. . 91 . 3 . 979–83 . 1994 . 8302877 . 10.1073/pnas.91.3.979 . 521437 . 1994PNAS...91..979T . etal. free .
• Bienroth S, Keller W, Wahle E . Assembly of a processive messenger RNA polyadenylation complex. . EMBO J. . 12 . 2 . 585–94 . 1993 . 8440247 . 10.1002/j.1460-2075.1993.tb05690.x. 413241 .
• Schul W, Groenhout B, Koberna K . The RNA 3' cleavage factors CstF 64 kDa and CPSF 100 kDa are concentrated in nuclear domains closely associated with coiled bodies and newly synthesized RNA. . EMBO J. . 15 . 11 . 2883–92 . 1996 . 8654386 . 10.1002/j.1460-2075.1996.tb00649.x. 450226 . etal.
• McCracken S, Fong N, Yankulov K . The C-terminal domain of RNA polymerase II couples mRNA processing to transcription. . Nature . 385 . 6614 . 357–61 . 1997 . 9002523 . 10.1038/385357a0 . 1997Natur.385..357M . 4307073 . etal.
• Takagaki Y, Manley JL . Complex protein interactions within the human polyadenylation machinery identify a novel component. . Mol. Cell. Biol. . 20 . 5 . 1515–25 . 2000 . 10669729 . 10.1128/MCB.20.5.1515-1525.2000 . 85326 .
• Nagase T, Kikuno R, Ishikawa KI . Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro. . DNA Res. . 7 . 1 . 65–73 . 2000 . 10718198 . 10.1093/dnares/7.1.65 . etal. free .
• Monsalve M, Wu Z, Adelmant G . Direct coupling of transcription and mRNA processing through the thermogenic coactivator PGC-1. . Mol. Cell . 6 . 2 . 307–16 . 2000 . 10983978 . 10.1016/S1097-2765(00)00031-9 . etal. free .
• de Vries H, Rüegsegger U, Hübner W . Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors. . EMBO J. . 19 . 21 . 5895–904 . 2000 . 11060040 . 10.1093/emboj/19.21.5895 . 305781 . etal.
• Hodgman R, Tay J, Mendez R, Richter JD . CPEB phosphorylation and cytoplasmic polyadenylation are catalyzed by the kinase IAK1/Eg2 in maturing mouse oocytes. . Development . 128 . 14 . 2815–22 . 2001 . 10.1242/dev.128.14.2815 . 11526086 .
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Ota T, Suzuki Y, Nishikawa T . Complete sequencing and characterization of 21,243 full-length human cDNAs. . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . etal. free .
• Kaufmann I, Martin G, Friedlein A . Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase. . EMBO J. . 23 . 3 . 616–26 . 2005 . 14749727 . 10.1038/sj.emboj.7600070 . 1271804 . etal.
• Beausoleil SA, Jedrychowski M, Schwartz D . Large-scale characterization of HeLa cell nuclear phosphoproteins. . Proc. Natl. Acad. Sci. U.S.A. . 101 . 33 . 12130–5 . 2004 . 15302935 . 10.1073/pnas.0404720101 . 514446 . 2004PNAS..10112130B . etal. free .
• Gerhard DS, Wagner L, Feingold EA . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Kimura K, Wakamatsu A, Suzuki Y . Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. . Genome Res. . 16 . 1 . 55–65 . 2006 . 16344560 . 10.1101/gr.4039406 . 1356129 . etal.
• Olsen JV, Blagoev B, Gnad F . Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. . Cell . 127 . 3 . 635–48 . 2006 . 17081983 . 10.1016/j.cell.2006.09.026 . etal. free .
• Ewing RM, Chu P, Elisma F . Large-scale mapping of human protein-protein interactions by mass spectrometry. . Mol. Syst. Biol. . 3 . 1. 89 . 2007 . 17353931 . 10.1038/msb4100134 . 1847948 . etal.

Notes and References

1. Jenny A, Hauri HP, Keller W . Characterization of cleavage and polyadenylation specificity factor and cloning of its 100-kilodalton subunit . Mol Cell Biol . 14 . 12 . 8183–90 . Dec 1994 . 7969155 . 359357 . 10.1128/MCB.14.12.8183.
2. Samiotaki M, Balatsos NA, Courtis N, Tsiapalis CM . Assignment of the 100-kDa subunit of cleavage and polyadenylation specificity factor (CPSF2) to human chromosome 14q31.3 by radiation hybrid mapping . Cytogenet Cell Genet . 90 . 3–4 . 328–9 . Jan 2001 . 11124543 . 10.1159/000056798 . 84335742 .
3. Web site: Entrez Gene: CPSF2 cleavage and polyadenylation specific factor 2, 100kDa.
4. Web site: Human Gene CPSF2 (ENST00000298875.9) from GENCODE V43 . 2023-03-05 . genome.ucsc.edu.
5. Zhang . Yixiao . Sun . Yadong . Shi . Yongsheng . Walz . Thomas . Tong . Liang . 2020-02-20 . Structural Insights into the Human Pre-mRNA 3′-End Processing Machinery . Molecular Cell . English . 77 . 4 . 800–809.e6 . 10.1016/j.molcel.2019.11.005 . 1097-2765 . 7036032 . 31810758.