CPD (gene) explained

Carboxypeptidase D is an enzyme that in humans is encoded by the CPD gene.^{[1] [2]}

Function

The metallocarboxypeptidase family of enzymes is divided into 2 subfamilies based on sequence similarities: the pancreatic carboxypeptidase-like and the regulatory B-type carboxypeptidase subfamilies. Carboxypeptidase D has been identified as a regulatory B-type carboxypeptidase. CPD is a homolog of duck gp180, a hepatitis B virus binding protein. Transcript variants utilizing alternative polyadenylation signals exist for this gene.^[3]

Further reading

• Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA . A "double adaptor" method for improved shotgun library construction . Analytical Biochemistry . 236 . 1 . 107–13 . Apr 1996 . 8619474 . 10.1006/abio.1996.0138 .
• McGwire GB, Tan F, Michel B, Rehli M, Skidgel RA . Identification of a membrane-bound carboxypeptidase as the mammalian homolog of duck gp180, a hepatitis B virus-binding protein . Life Sciences . 60 . 10 . 715–24 . 1997 . 9064476 . 10.1016/S0024-3205(96)00642-X .
• Varlamov O, Fricker LD . Intracellular trafficking of metallocarboxypeptidase D in AtT-20 cells: localization to the trans-Golgi network and recycling from the cell surface . Journal of Cell Science . 111 (Pt 7) . 7 . 877–85 . Apr 1998 . 10.1242/jcs.111.7.877 . 9490632 .
• Ishikawa T, Murakami K, Kido Y, Ohnishi S, Yazaki Y, Harada F, Kuroki K . Cloning, functional expression, and chromosomal localization of the human and mouse gp180-carboxypeptidase D-like enzyme . Gene . 215 . 2 . 361–70 . Jul 1998 . 9714835 . 10.1016/S0378-1119(98)00270-4 .
• Reznik SE, Salafia CM, Lage JM, Fricker LD . Immunohistochemical localization of carboxypeptidases E and D in the human placenta and umbilical cord . The Journal of Histochemistry and Cytochemistry . 46 . 12 . 1359–68 . Dec 1998 . 9815277 . 10.1177/002215549804601204 . free .
• Hadkar V, Skidgel RA . Carboxypeptidase D is up-regulated in raw 264.7 macrophages and stimulates nitric oxide synthesis by cells in arginine-free medium . Molecular Pharmacology . 59 . 5 . 1324–32 . May 2001 . 11306718 . 10.1124/mol.59.5.1324. 10456432 .
• Fan X, Olson SJ, Blevins LS, Allen GS, Johnson MD . Immunohistochemical localization of carboxypeptidases D, E, and Z in pituitary adenomas and normal human pituitary . The Journal of Histochemistry and Cytochemistry . 50 . 11 . 1509–16 . Nov 2002 . 12417617 . 10.1177/002215540205001111 . free .
• Kalinina EV, Fricker LD . Palmitoylation of carboxypeptidase D. Implications for intracellular trafficking . The Journal of Biological Chemistry . 278 . 11 . 9244–9 . Mar 2003 . 12643288 . 10.1074/jbc.M209379200 . free .
• Zhang H, Li XJ, Martin DB, Aebersold R . Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry . Nature Biotechnology . 21 . 6 . 660–6 . Jun 2003 . 12754519 . 10.1038/nbt827 . 581283 .
• O'Malley PG, Sangster SM, Abdelmagid SA, Bearne SL, Too CK . Characterization of a novel, cytokine-inducible carboxypeptidase D isoform in haematopoietic tumour cells . The Biochemical Journal . 390 . Pt 3 . 665–73 . Sep 2005 . 15918796 . 1199659 . 10.1042/BJ20050025 .

Notes and References

1. Riley DA, Tan F, Miletich DJ, Skidgel RA . Chromosomal localization of the genes for human carboxypeptidase D (CPD) and the active 50-kilodalton subunit of human carboxypeptidase N (CPN1) . Genomics . 50 . 1 . 105–8 . May 1998 . 9628828 . 10.1006/geno.1998.5295 .
2. Tan F, Rehli M, Krause SW, Skidgel RA . Sequence of human carboxypeptidase D reveals it to be a member of the regulatory carboxypeptidase family with three tandem active site domains . The Biochemical Journal . 327 (Pt 1) . Pt 1 . 81–7 . Oct 1997 . 9355738 . 1218766 . 10.1042/bj3270081.
3. Web site: Entrez Gene: CPD carboxypeptidase D.