CNOT7 explained

CCR4-NOT transcription complex subunit 7 is a protein that in humans is encoded by the CNOT7 gene.^{[1] [2] [3] [4]} It is a subunit of the CCR4-Not deadenylase complex.

Function

The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The protein has both mouse and yeast orthologs. Alternate splicing of this gene results in two transcript variants encoding different isoforms.^[4]

Interactions

CNOT7 has been shown to interact with:

• BTG1,^{[5] [6]}
• PABPC1,
• TOB1,^[7] and
• TOB2.^[8]

Further reading

• Draper MP, Salvadore C, Denis CL . Identification of a mouse protein whose homolog in Saccharomyces cerevisiae is a component of the CCR4 transcriptional regulatory complex . Mol. Cell. Biol. . 15 . 7 . 3487–95 . 1995 . 7791755 . 230585 . 10.1128/MCB.15.7.3487.
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Hillier LD, Lennon G, Becker M, Bonaldo MF, Chiapelli B, Chissoe S, Dietrich N, DuBuque T, Favello A, Gish W, Hawkins M, Hultman M, Kucaba T, Lacy M, Le M, Le N, Mardis E, Moore B, Morris M, Parsons J, Prange C, Rifkin L, Rohlfing T, Schellenberg K, Bento Soares M, Tan F, Thierry-Meg J, Trevaskis E, Underwood K, Wohldman P, Waterston R, Wilson R, Marra M . Generation and analysis of 280,000 human expressed sequence tags . Genome Res. . 6 . 9 . 807–28 . 1996 . 8889549 . 10.1101/gr.6.9.807 . free .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Rouault JP, Prévôt D, Berthet C, Birot AM, Billaud M, Magaud JP, Corbo L . Interaction of BTG1 and p53-regulated BTG2 gene products with mCaf1, the murine homolog of a component of the yeast CCR4 transcriptional regulatory complex . J. Biol. Chem. . 273 . 35 . 22563–9 . 1998 . 9712883 . 10.1074/jbc.273.35.22563 . free .
• Bogdan JA, Adams-Burton C, Pedicord DL, Sukovich DA, Benfield PA, Corjay MH, Stoltenborg JK, Dicker IB . Human carbon catabolite repressor protein (CCR4)-associative factor 1: cloning, expression and characterization of its interaction with the B-cell translocation protein BTG1 . Biochem. J. . 336 . 2 . 471–81 . 1998 . 9820826 . 1219893 . 10.1042/bj3360471.
• Ikematsu N, Yoshida Y, Kawamura-Tsuzuku J, Ohsugi M, Onda M, Hirai M, Fujimoto J, Yamamoto T . Tob2, a novel anti-proliferative Tob/BTG1 family member, associates with a component of the CCR4 transcriptional regulatory complex capable of binding cyclin-dependent kinases . Oncogene . 18 . 52 . 7432–41 . 1999 . 10602502 . 10.1038/sj.onc.1203193 . free .
• Prévôt D, Morel AP, Voeltzel T, Rostan MC, Rimokh R, Magaud JP, Corbo L . Relationships of the antiproliferative proteins BTG1 and BTG2 with CAF1, the human homolog of a component of the yeast CCR4 transcriptional complex: involvement in estrogen receptor alpha signaling pathway . J. Biol. Chem. . 276 . 13 . 9640–8 . 2001 . 11136725 . 10.1074/jbc.M008201200 . free .
• Yoshida Y, Hosoda E, Nakamura T, Yamamoto T . Association of ANA, a member of the antiproliferative Tob family proteins, with a Caf1 component of the CCR4 transcriptional regulatory complex . Jpn. J. Cancer Res. . 92 . 6 . 592–6 . 2001 . 11429045 . 10.1111/j.1349-7006.2001.tb01135.x . 5926753 .
• Flanagan J, Healey S, Young J, Whitehall V, Chenevix-Trench G . Analysis of the transcription regulator, CNOT7, as a candidate chromosome 8 tumor suppressor gene in colorectal cancer . Int. J. Cancer . 106 . 4 . 505–9 . 2003 . 12845644 . 10.1002/ijc.11264 . 25191544 . free .
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
• Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S . Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes . Genome Res. . 16 . 1 . 55–65 . 2006 . 16344560 . 1356129 . 10.1101/gr.4039406 .

Notes and References

1. Albert TK, Lemaire M, van Berkum NL, Gentz R, Collart MA, Timmers HT . Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits . Nucleic Acids Res. . 28 . 3 . 809–17 . March 2000 . 10637334 . 102560 . 10.1093/nar/28.3.809 .
2. Adams MD, Dubnick M, Kerlavage AR, Moreno R, Kelley JM, Utterback TR, Nagle JW, Fields C, Venter JC . Sequence identification of 2,375 human brain genes . Nature . 355 . 6361 . 632–4 . March 1992 . 1538749 . 10.1038/355632a0 . 1992Natur.355..632A . 4234345 .
3. Robin-Lespinasse Y, Sentis S, Kolytcheff C, Rostan MC, Corbo L, Le Romancer M . hCAF1, a new regulator of PRMT1-dependent arginine methylation . J. Cell Sci. . 120 . Pt 4 . 638–47 . February 2007 . 17264152 . 10.1242/jcs.03357 . 6592326 .
4. Web site: Entrez Gene: CNOT7 CCR4-NOT transcription complex, subunit 7.
5. Bogdan JA, Adams-Burton C, Pedicord DL, Sukovich DA, Benfield PA, Corjay MH, Stoltenborg JK, Dicker IB . Human carbon catabolite repressor protein (CCR4)-associative factor 1: cloning, expression and characterization of its interaction with the B-cell translocation protein BTG1 . Biochem. J. . 336 . 2. 471–81 . December 1998 . 9820826 . 1219893 . 10.1042/bj3360471.
6. Prévôt D, Morel AP, Voeltzel T, Rostan MC, Rimokh R, Magaud JP, Corbo L . Relationships of the antiproliferative proteins BTG1 and BTG2 with CAF1, the human homolog of a component of the yeast CCR4 transcriptional complex: involvement in estrogen receptor alpha signaling pathway . J. Biol. Chem. . 276 . 13 . 9640–8 . March 2001 . 11136725 . 10.1074/jbc.M008201200 . free .
7. Funakoshi Y, Doi Y, Hosoda N, Uchida N, Osawa M, Shimada I, Tsujimoto M, Suzuki T, Katada T, Hoshino S . Mechanism of mRNA deadenylation: evidence for a molecular interplay between translation termination factor eRF3 and mRNA deadenylases . Genes Dev. . 21 . 23 . 3135–48 . December 2007 . 18056425 . 2081979 . 10.1101/gad.1597707 .
8. Ikematsu N, Yoshida Y, Kawamura-Tsuzuku J, Ohsugi M, Onda M, Hirai M, Fujimoto J, Yamamoto T . Tob2, a novel anti-proliferative Tob/BTG1 family member, associates with a component of the CCR4 transcriptional regulatory complex capable of binding cyclin-dependent kinases . Oncogene . 18 . 52 . 7432–41 . December 1999 . 10602502 . 10.1038/sj.onc.1203193 . free .