CMP kinase explained

UMP-CMP kinase is an enzyme that in humans is encoded by the CMPK1 gene.^[1]

Function

Uridine monophosphate (UMP)/cytidine monophosphate (CMP) kinase (EC 2.7.4.4) catalyzes the phosphoryl transfer from ATP to UMP, CMP, and deoxy-CMP (dCMP), resulting in the formation of ADP and the corresponding nucleoside diphosphate. These nucleoside diphosphates are required for cellular nucleic acid synthesis.^[2]

Further reading

• Hochstrasser DF, Frutiger S, Paquet N, Bairoch A, Ravier F, Pasquali C, Sanchez JC, Tissot JD, Bjellqvist B, Vargas R . Human liver protein map: A reference database established by microsequencing and gel comparison . Electrophoresis . 13 . 12 . 992–1001 . 1992 . 1286669 . 10.1002/elps.11501301201 . 23518983 .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Hughes GJ, Frutiger S, Paquet N, Pasquali C, Sanchez JC, Tissot JD, Bairoch A, Appel RD, Hochstrasser DF . Human liver protein map: Update 1993 . Electrophoresis . 14 . 11 . 1216–22 . 1993 . 8313870 . 10.1002/elps.11501401181 . 33424554 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S . Construction and characterization of a full length-enriched and a 5′-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Hu RM, Han ZG, Song HD, Peng YD, Huang QH, Ren SX, Gu YJ, Huang CH, Li YB, Jiang CL, Fu G, Zhang QH, Gu BW, Dai M, Mao YF, Gao GF, Rong R, Ye M, Zhou J, Xu SH, Gu J, Shi JX, Jin WR, Zhang CK, Wu TM, Huang GY, Chen Z, Chen MD, Chen JL . Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning . Proceedings of the National Academy of Sciences . 97 . 17 . 9543–8 . 2000 . 10931946 . 16901 . 10.1073/pnas.160270997 . 2000PNAS...97.9543H . free .
• Pearman AT, Castro-Faria-Neto HC, McIntyre TM, Prescott SM, Stafforini DM . Characterization of human UMP-CMP kinase enzymatic activity and 5′ untranslated region . Life Sciences . 69 . 20 . 2361–70 . 2001 . 11681623 . 10.1016/S0024-3205(01)01322-4 .
• Liou JY, Dutschman GE, Lam W, Jiang Z, Cheng YC . Characterization of human UMP/CMP kinase and its phosphorylation of D- and L-form deoxycytidine analogue monophosphates . Cancer Research . 62 . 6 . 1624–31 . 2002 . 11912132 .
• Pasti C, Gallois-Montbrun S, Munier-Lehmann H, Veron M, Gilles AM, Deville-Bonne D . Reaction of human UMP-CMP kinase with natural and analog substrates . European Journal of Biochemistry . 270 . 8 . 1784–90 . 2003 . 12694191 . 10.1046/j.1432-1033.2003.03537.x . free .
• Segura-Peña D, Sekulic N, Ort S, Konrad M, Lavie A . Substrate-induced Conformational Changes in Human UMP/CMP Kinase . Journal of Biological Chemistry . 279 . 32 . 33882–9 . 2004 . 15163660 . 10.1074/jbc.M401989200 . free . 11858/00-001M-0000-0012-EC87-4 . free .
• Hsu CH, Liou JY, Dutschman GE, Cheng YC . Phosphorylation of Cytidine, Deoxycytidine, and Their Analog Monophosphates by Human UMP/CMP Kinase is Differentially Regulated by ATP and Magnesium . Molecular Pharmacology . 67 . 3 . 806–14 . 2004 . 15550676 . 10.1124/mol.104.006098 . 579635 .
• Wickham L, Benjannet S, Marcinkiewicz E, Chretien M, Seidah NG . Beta-Amyloid protein converting enzyme 1 and brain-specific type II membrane protein BRI3: binding partners processed by furin . Journal of Neurochemistry . 92 . 1 . 93–102 . 2005 . 15606899 . 10.1111/j.1471-4159.2004.02840.x . free .
• Topalis D, Kumamoto H, Amaya Velasco MF, Dugué L, Haouz A, Alexandre JA, Gallois-Montbrun S, Alzari PM, Pochet S, Agrofoglio LA, Deville-Bonne D . Nucleotide binding to human UMP-CMP kinase using fluorescent derivatives – a screening based on affinity for the UMP-CMP binding site . FEBS Journal . 274 . 14 . 3704–14 . 2007 . 17608725 . 10.1111/j.1742-4658.2007.05902.x . free .
• Gagyi C, Bucurenci N, Sîrbu O, Labesse G, Ionescu M, Ofiteru A, Assairi L, Landais S, Danchin A, Bârzu O, Gilles AM . UMP kinase from the Gram-positive bacterium Bacillus subtilis is strongly dependent on GTP for optimal activity . European Journal of Biochemistry . 270 . 15 . 3196–204 . 2003 . 12869195 . 10.1046/j.1432-1033.2003.03702.x . free .
• Ofiteru A, Bucurenci N, Alexov E, Bertrand T, Briozzo P, Munier-Lehmann H, Gilles AM . Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase . The FEBS Journal . 274 . 13 . 3363–73 . 2007 . 17542990 . 10.1111/j.1742-4658.2007.05870.x . 2394831 . free .

Notes and References

1. Van Rompay AR, Johansson M, Karlsson A . Phosphorylation of deoxycytidine analog monophosphates by UMP-CMP kinase: molecular characterization of the human enzyme . Molecular Pharmacology . 56 . 3 . 562–9 . 1999 . 10462544 . 10.1124/mol.56.3.562 . 22945276 .
2. Liou JY, Dutschman GE, Lam W, Jiang Z, Cheng YC . Characterization of human UMP/CMP kinase and its phosphorylation of D- and L-form deoxycytidine analogue monophosphates . Cancer Research . 62 . 6 . 1624–31 . 2002 . 11912132 .