CLCA1 explained

Chloride channel accessory 1 is a protein that in humans is encoded by the CLCA1 gene.^[1]

This gene encodes a member of the calcium sensitive chloride conductance protein family. To date, all members of this gene family map to the same region on chromosome 1p31-p22 and share a high degree of homology in size, sequence, and predicted structure, but differ significantly in their tissue distributions. The encoded protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits, although the site at which the precursor is cleaved has not been precisely determined. The encoded protein may be involved in mediating calcium-activated chloride conductance in the intestine.^[2] Protein structure prediction methods suggest the N-terminal region of CLCA1 protein is a zinc metalloprotease.^[3]

See also

• Chloride channel

Further reading

• Hume JR, Duan D, Collier ML . Anion transport in heart . Physiol. Rev. . 80 . 1 . 31–81 . 2000 . 10.1152/physrev.2000.80.1.31 . 10617765 . 15954128 . etal. free .
• Pauli BU, Abdel-Ghany M, Cheng HC . Molecular characteristics and functional diversity of CLCA family members . Clin. Exp. Pharmacol. Physiol. . 27 . 11 . 901–5 . 2001 . 11071307 . 10.1046/j.1440-1681.2000.03358.x . 28762005 . etal.
• Agnel M, Vermat T, Culouscou JM . Identification of three novel members of the calcium-dependent chloride channel (CaCC) family predominantly expressed in the digestive tract and trachea . FEBS Lett. . 455 . 3 . 295–301 . 1999 . 10437792 . 10.1016/S0014-5793(99)00891-1 . 82094058 . free .
• Dias Neto E, Correa RG, Verjovski-Almeida S . Shotgun sequencing of the human transcriptome with ORF expressed sequence tags . Proc. Natl. Acad. Sci. U.S.A. . 97 . 7 . 3491–6 . 2000 . 10737800 . 10.1073/pnas.97.7.3491 . 16267 . 2000PNAS...97.3491D . etal. free .
• Liu QH, Williams DA, McManus C . HIV-1 gp120 and chemokines activate ion channels in primary macrophages through CCR5 and CXCR4 stimulation . Proc. Natl. Acad. Sci. U.S.A. . 97 . 9 . 4832–7 . 2000 . 10758170 . 10.1073/pnas.090521697 . 18318 . 2000PNAS...97.4832L . etal. free .
• Toda M, Tulic MK, Levitt RC, Hamid Q . A calcium-activated chloride channel (HCLCA1) is strongly related to IL-9 expression and mucus production in bronchial epithelium of patients with asthma . J. Allergy Clin. Immunol. . 109 . 2 . 246–50 . 2002 . 11842292 . 10.1067/mai.2002.121555 . free .
• Greenwood IA, Miller LJ, Ohya S, Horowitz B . The large conductance potassium channel beta-subunit can interact with and modulate the functional properties of a calcium-activated chloride channel, CLCA1 . J. Biol. Chem. . 277 . 25 . 22119–22 . 2002 . 11994272 . 10.1074/jbc.C200215200 . free .
• Abdel-Ghany M, Cheng HC, Elble RC, Pauli BU . Focal adhesion kinase activated by beta(4) integrin ligation to mCLCA1 mediates early metastatic growth . J. Biol. Chem. . 277 . 37 . 34391–400 . 2002 . 12110680 . 10.1074/jbc.M205307200 . free .
• Loewen ME, Bekar LK, Gabriel SE . pCLCA1 becomes a cAMP-dependent chloride conductance mediator in Caco-2 cells . Biochem. Biophys. Res. Commun. . 298 . 4 . 531–6 . 2002 . 12408984 . 10.1016/S0006-291X(02)02498-1 . etal.
• Book: Zhou Y, Shapiro M, Dong Q . Mucus Hypersecretion in Respiratory Disease . A calcium-activated chloride channel blocker inhibits goblet cell metaplasia and mucus overproduction . Novartis Foundation Symposia . 248 . 150–65; discussion 165–70, 277–82 . 2003 . 12568493 . 10.1002/0470860790.ch10 . 9780470844786 . etal.
• Abdel-Ghany M, Cheng HC, Elble RC . The interacting binding domains of the beta(4) integrin and calcium-activated chloride channels (CLCAs) in metastasis . J. Biol. Chem. . 278 . 49 . 49406–16 . 2004 . 14512419 . 10.1074/jbc.M309086200 . etal. free .
• Ota T, Suzuki Y, Nishikawa T . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . etal. free .
• Hegab AE, Sakamoto T, Uchida Y . CLCA1 gene polymorphisms in chronic obstructive pulmonary disease . J. Med. Genet. . 41 . 3 . 27e–27 . 2004 . 14985398 . 10.1136/jmg.2003.012484 . 1735703 . etal.
• Kamada F, Suzuki Y, Shao C . Association of the hCLCA1 gene with childhood and adult asthma . Genes Immun. . 5 . 7 . 540–7 . 2005 . 15318163 . 10.1038/sj.gene.6364124 . etal. free .
• Ritzka M, Stanke F, Jansen S . The CLCA gene locus as a modulator of the gastrointestinal basic defect in cystic fibrosis . Hum. Genet. . 115 . 6 . 483–91 . 2005 . 15490240 . 10.1007/s00439-004-1190-y . 12935280 . etal.
• Gibson A, Lewis AP, Affleck K . hCLCA1 and mCLCA3 are secreted non-integral membrane proteins and therefore are not ion channels . J. Biol. Chem. . 280 . 29 . 27205–12 . 2005 . 15919655 . 10.1074/jbc.M504654200 . etal. free .
• Jeong SM, Park HK, Yoon IS . Cloning and expression of Ca²⁺-activated chloride channel from rat brain . Biochem. Biophys. Res. Commun. . 334 . 2 . 569–76 . 2005 . 16023076 . 10.1016/j.bbrc.2005.06.122 . etal.

Notes and References

1. Gruber AD, Elble RC, Ji HL, Schreur KD, Fuller CM, Pauli BU . Genomic cloning, molecular characterization, and functional analysis of human CLCA1, the first human member of the family of Ca²⁺-activated Cl⁻ channel proteins . Genomics . 54 . 2 . 200–14 . Jan 1999 . 9828122 . 10.1006/geno.1998.5562 . free .
2. Web site: Entrez Gene: CLCA1 chloride channel, calcium activated, family member 1.
3. Pawłowski K, Lepistö M, Meinander N . Novel conserved hydrolase domain in the CLCA family of alleged calcium-activated chloride channels . Proteins . 63 . 3 . 424–39 . 2006 . 16470849. 10.1002/prot.20887. 40041491 . etal.