CLASP1 explained

Cytoplasmic linker associated protein 1, also known as CLASP1, is a protein which in humans is encoded by the CLASP1 gene.^[1]

Function

CLASP1 belongs to a family of microtubule-associated proteins involved in attachment of microtubules to the cell cortex in animals^[2] and plants.^[3] CLASPs, such as CLASP1, interact with CLIPs (e.g., CLIP1). In animal cells, CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle.^[4] CLASP1 controls the interactions of astral microtubules with the cell cortex in mitosis, which is important for the proper positioning and orientation of the spindle.^[5]

Further reading

• Ambrose JC, Wasteneys GO . CLASP Modulates Microtubule-Cortex Interaction during Self-Organization of Acentrosomal Microtubules . Mol Biol Cell . 19 . 4730–4737 . 2008 . 18716054 . 10.1091/mbc.E08-06-0665 . 11 . 2575154.
• Galjart N . CLIPs and CLASPs and cellular dynamics . Nat. Rev. Mol. Cell Biol. . 6 . 6 . 487–98 . 2005 . 15928712 . 10.1038/nrm1664 . 23216754 .
• Ishikawa K, Nagase T, Suyama M . Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro . DNA Res. . 5 . 3 . 169–76 . 1998 . 9734811 . 10.1093/dnares/5.3.169 . etal. free .
• Lemos CL, Sampaio P, Maiato H . Mast, a conserved microtubule-associated protein required for bipolar mitotic spindle organization . EMBO J. . 19 . 14 . 3668–82 . 2000 . 10899121 . 10.1093/emboj/19.14.3668 . 313969 . etal.
• Casper Hoogenraad . Akhmanova A, Hoogenraad CC, Drabek K . Clasps are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts . Cell . 104 . 6 . 923–35 . 2001 . 11290329 . 10.1016/S0092-8674(01)00288-4 . etal. free .
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Maiato H, Fairley EA, Rieder CL . Human CLASP1 is an outer kinetochore component that regulates spindle microtubule dynamics . Cell . 113 . 7 . 891–904 . 2003 . 12837247 . 10.1016/S0092-8674(03)00465-3 . etal. free . 10216/53832 . free .
• Maiato H, Rieder CL, Earnshaw WC, Sunkel CE . How do kinetochores CLASP dynamic microtubules? . Cell Cycle . 2 . 6 . 511–4 . 2004 . 14504462 . 10.4161/cc.2.6.576 . free . 10216/35062 . free .
• Ota T, Suzuki Y, Nishikawa T . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . etal. free .
• Beausoleil SA, Jedrychowski M, Schwartz D . Large-scale characterization of HeLa cell nuclear phosphoproteins . Proc. Natl. Acad. Sci. U.S.A. . 101 . 33 . 12130–5 . 2004 . 15302935 . 10.1073/pnas.0404720101 . 514446 . 2004PNAS..10112130B . etal. free .
• Jin J, Smith FD, Stark C . Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization . Curr. Biol. . 14 . 16 . 1436–50 . 2004 . 15324660 . 10.1016/j.cub.2004.07.051 . etal. free . 2004CBio...14.1436J .
• Gerhard DS, Wagner L, Feingold EA . The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Mimori-Kiyosue Y, Grigoriev I, Lansbergen G . CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex . J. Cell Biol. . 168 . 1 . 141–53 . 2005 . 15631994 . 10.1083/jcb.200405094 . 2171674 . etal.
• Hillier LW, Graves TA, Fulton RS . Generation and annotation of the DNA sequences of human chromosomes 2 and 4 . Nature . 434 . 7034 . 724–31 . 2005 . 15815621 . 10.1038/nature03466 . 2005Natur.434..724H . etal. free .
• Venables JP, Bourgeois CF, Dalgliesh C . Up-regulation of the ubiquitous alternative splicing factor Tra2beta causes inclusion of a germ cell-specific exon . Hum. Mol. Genet. . 14 . 16 . 2289–303 . 2005 . 16000324 . 10.1093/hmg/ddi233 . etal.
• Aonuma M, Miyamoto M, Inoue YH . Microtubule bundle formation and cell death induced by the human CLASP/Orbit N-terminal fragment . Cell Struct. Funct. . 30 . 1 . 7–13 . 2006 . 16145243 . 10.1247/csf.30.7 . etal. free .
• Lansbergen G, Grigoriev I, Mimori-Kiyosue Y . CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta . Dev. Cell . 11 . 1 . 21–32 . 2006 . 16824950 . 10.1016/j.devcel.2006.05.012 . etal. free .
• Mimori-Kiyosue Y, Grigoriev I, Sasaki H . Mammalian CLASPs are required for mitotic spindle organization and kinetochore alignment . Genes Cells . 11 . 8 . 845–57 . 2006 . 16866869 . 10.1111/j.1365-2443.2006.00990.x . 33744175 . etal. free .
• Pereira AL, Pereira AJ, Maia AR . Mammalian CLASP1 and CLASP2 Cooperate to Ensure Mitotic Fidelity by Regulating Spindle and Kinetochore Function . Mol. Biol. Cell . 17 . 10 . 4526–42 . 2007 . 16914514 . 10.1091/mbc.E06-07-0579 . 1635371 . etal.
• Ewing RM, Chu P, Elisma F . Large-scale mapping of human protein–protein interactions by mass spectrometry . Mol. Syst. Biol. . 3 . 1. 89 . 2007 . 17353931 . 10.1038/msb4100134 . 1847948 . etal.

Notes and References

1. Web site: Entrez Gene: CLASP1 cytoplasmic linker associated protein 1.
2. Lansbergen G, Grigoriev I, Mimori-Kiyosue Y. CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5beta . Developmental Cell. 2006. 11 . 21–32. 16824950. 10.1016/j.devcel.2006.05.012. 1 . etal. free.
3. Ambrose JC, Wasteneys GO. CLASP Modulates Microtubule-Cortex Interaction during Self-Organization of Acentrosomal Microtubules . Molecular Biology of the Cell. 2008. 19 . 4730–4737. 18716054. 10.1091/mbc.E08-06-0665. 11. 2575154.
4. Maiato H, Fairley EA, Rieder CL, Swedlow JR, Sunkel CE, Earnshaw WC . Human CLASP1 is an outer kinetochore component that regulates spindle microtubule dynamics . Cell . 113 . 7 . 891–904 . June 2003 . 12837247 . 10.1016/S0092-8674(03)00465-3. free . 10216/53832 . free .
5. Samora. Catarina P.. Mogessie. Binyam. Conway. Leslie. Ross. Jennifer L.. Straube. Anne. McAinsh. Andrew D.. MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis. Nature Cell Biology. 7 August 2011. 13. 9. 1040–1050. 10.1038/ncb2297. 21822276. 8869880.