CKMT1B explained

Creatine kinase, mitochondrial 1B also known as CKMT1B is one of two genes which encode the ubiquitous mitochondrial creatine kinase (ubiquitous mtCK or CKMT1).

Function

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK (CKMT2) and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 (CKMT1A and CKMT1B (this gene)) have been identified which encode identical mitochondrial creatine kinase proteins.^[1]

Clinical significance

Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis.^[1]

Further reading

• Payne RM, Strauss AW . Expression of the mitochondrial creatine kinase genes . Mol. Cell. Biochem. . 133-134 . 235–43 . 1995 . 7808456 . 10.1007/BF01267957 . 24795492 .
• Qin W, Khuchua Z, Cheng J, Boero J, Payne RM, Strauss AW . Molecular characterization of the creatine kinases and some historical perspectives . Mol. Cell. Biochem. . 184 . 1–2 . 153–67 . 1998 . 9746319 . 10.1023/A:1006807515892 . 1577790 .
• Haas RC, Strauss AW . Separate nuclear genes encode sarcomere-specific and ubiquitous human mitochondrial creatine kinase isoenzymes . J. Biol. Chem. . 265 . 12 . 6921–7 . 1990 . 10.1016/S0021-9258(19)39237-3 . 2324105 . free .
• Haas RC, Korenfeld C, Zhang ZF, Perryman B, Roman D, Strauss AW . Isolation and characterization of the gene and cDNA encoding human mitochondrial creatine kinase . J. Biol. Chem. . 264 . 5 . 2890–7 . 1989 . 10.1016/S0021-9258(19)81696-4 . 2914937 . free .
• Stallings RL, Olson E, Strauss AW, Thompson LH, Bachinski LL, Siciliano MJ . Human creatine kinase genes on chromosomes 15 and 19, and proximity of the gene for the muscle form to the genes for apolipoprotein C2 and excision repair . Am. J. Hum. Genet. . 43 . 2 . 144–51 . 1988 . 3400641 . 1715361 .
• Jacobus WE, Moreadith RW, Vandegaer KM . Control of heart oxidative phosphorylation by creatine kinase in mitochondrial membranes . Ann. N. Y. Acad. Sci. . 414 . 1. 73–89 . 1984 . 6584077 . 10.1111/j.1749-6632.1983.tb31676.x . 1983NYASA.414...73J . 7881554 .
• Brdiczka D, Kaldis P, Wallimann T . In vitro complex formation between the octamer of mitochondrial creatine kinase and porin . J. Biol. Chem. . 269 . 44 . 27640–4 . 1994 . 10.1016/S0021-9258(18)47033-0 . 7525559 . free .
• Steeghs K, Merkx G, Wieringa B . The ubiquitous mitochondrial creatine kinase gene maps to a conserved region on human chromosome 15q15 and mouse chromosome 2 bands F1-F3 . Genomics . 24 . 1 . 193–5 . 1995 . 7896282 . 10.1006/geno.1994.1604 .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Fritz-Wolf K, Schnyder T, Wallimann T, Kabsch W . Structure of mitochondrial creatine kinase . Nature . 381 . 6580 . 341–5 . 1996 . 8692275 . 10.1038/381341a0 . 1996Natur.381..341F . 4254253 .
• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery . Genome Res. . 6 . 9 . 791–806 . 1997 . 8889548 . 10.1101/gr.6.9.791 . free .
• Thomure MF, Gast MJ, Srivastava N, Payne RM . Regulation of creatine kinase isoenzymes in human placenta during early, mid-, and late gestation . J. Soc. Gynecol. Investig. . 3 . 6 . 322–7 . 1997 . 8923416 . 10.1016/S1071-5576(96)00043-3 . 7814307 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 .
• Eder M, Fritz-Wolf K, Kabsch W, Wallimann T, Schlattner U . Crystal structure of human ubiquitous mitochondrial creatine kinase . Proteins . 39 . 3 . 216–25 . 2000 . 10737943 . 10.1002/(SICI)1097-0134(20000515)39:3<216::AID-PROT40>3.0.CO;2-# . 12531664 .
• Schlattner U, Wallimann T . Octamers of mitochondrial creatine kinase isoenzymes differ in stability and membrane binding . J. Biol. Chem. . 275 . 23 . 17314–20 . 2000 . 10748055 . 10.1074/jbc.M001919200 . free .
• Schlattner U, Dolder M, Wallimann T, Tokarska-Schlattner M . Mitochondrial creatine kinase and mitochondrial outer membrane porin show a direct interaction that is modulated by calcium . J. Biol. Chem. . 276 . 51 . 48027–30 . 2002 . 11602586 . 10.1074/jbc.M106524200 . free .
• Schlattner U, Wallimann T . A quantitative approach to membrane binding of human ubiquitous mitochondrial creatine kinase using surface plasmon resonance . J. Bioenerg. Biomembr. . 32 . 1 . 123–31 . 2002 . 11768757 . 10.1023/A:1005576831968 . 24884381 . 20.500.11850/422769 . free .
• Schlattner U, Möckli N, Speer O, Werner S, Wallimann T . Creatine kinase and creatine transporter in normal, wounded, and diseased skin . J. Invest. Dermatol. . 118 . 3 . 416–23 . 2002 . 11874479 . 10.1046/j.0022-202x.2001.01697.x . free .

Notes and References

1. Web site: Entrez Gene: CKMT1B creatine kinase, mitochondrial 1B.