CKAP5 explained

Cytoskeleton-associated protein 5 is a microtubule-associated protein that in humans is encoded by the CKAP5 gene.^{[1] [2] [3]} It is the homolog of the Xenopus protein XMAP215^[4] and is also known as ch-Tog.

It has at least two distinct roles in spindle formation: it protects kinetochore microtubules from depolymerization by MCAK (KIF2C), and ch-Tog plays an essential role in centrosomal microtubule assembly, a function independent of MCAK activity.^[5]

Interactions

CKAP5 has been shown to interact with TACC1.^{[6] [7]}

Further reading

• Basto R, Gergely F, Draviam VM, Ohkura H, Liley K, Raff JW . Hsp90 is required to localise cyclin B and Msps/ch-TOG to the mitotic spindle in Drosophila and humans . J. Cell Sci. . 120 . Pt 7 . 1278–87 . 2007 . 17376965 . 10.1242/jcs.000604 . free .
• Kosturko LD, Maggipinto MJ, D'Sa C, Carson JH, Barbarese E . The microtubule-associated protein tumor overexpressed gene binds to the RNA trafficking protein heterogeneous nuclear ribonucleoprotein A2 . Mol. Biol. Cell . 16 . 4 . 1938–47 . 2005 . 15703215 . 1073673 . 10.1091/mbc.E04-08-0709 .
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP . Large-scale characterization of HeLa cell nuclear phosphoproteins . Proc. Natl. Acad. Sci. U.S.A. . 101 . 33 . 12130–5 . 2004 . 15302935 . 514446 . 10.1073/pnas.0404720101 . 2004PNAS..10112130B . free .
• Cassimeris L, Morabito J . TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly . Mol. Biol. Cell . 15 . 4 . 1580–90 . 2004 . 14718566 . 379257 . 10.1091/mbc.E03-07-0544 .
• Conte N, Delaval B, Ginestier C, Ferrand A, Isnardon D, Larroque C, Prigent C, Séraphin B, Jacquemier J, Birnbaum D . TACC1-chTOG-Aurora A protein complex in breast cancer . Oncogene . 22 . 50 . 8102–16 . 2003 . 14603251 . 10.1038/sj.onc.1206972 . 10536264 .
• Gergely F, Draviam VM, Raff JW . The ch-TOG/XMAP215 protein is essential for spindle pole organization in human somatic cells . Genes Dev. . 17 . 3 . 336–41 . 2003 . 12569123 . 195983 . 10.1101/gad.245603 .
• Nakayama M, Kikuno R, Ohara O . Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs . Genome Res. . 12 . 11 . 1773–84 . 2002 . 12421765 . 187542 . 10.1101/gr.406902 .
• Lauffart B, Howell SJ, Tasch JE, Cowell JK, Still IH . Interaction of the transforming acidic coiled-coil 1 (TACC1) protein with ch-TOG and GAS41/NuBI1 suggests multiple TACC1-containing protein complexes in human cells . Biochem. J. . 363 . Pt 1 . 195–200 . 2002 . 11903063 . 1222467 . 10.1042/0264-6021:3630195 .
• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery . Genome Res. . 6 . 9 . 791–806 . 1996 . 8889548 . 10.1101/gr.6.9.791 . free .
• Nakamura S, Grigoriev I, Nogi T, Hamaji T, Cassimeris L, Mimori-Kiyosue Y . Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells . PLOS ONE . 7 . 12 . e51442 . 2012 . 23251535 . 10.1371/journal.pone.0051442 . 3520847. 2012PLoSO...751442N . free .
• Zanic M, Widlund PO, Hyman AA, Howard J . Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels . Nat. Cell Biol. . 15 . 6 . 688–93 . 2013 . 23666085 . 10.1038/ncb2744 . 3025200 .

Notes and References

1. Nagase T, Miyajima N, Tanaka A, Sazuka T, Seki N, Sato S, Tabata S, Ishikawa K, Kawarabayasi Y, Kotani H . Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1 . DNA Res. . 2 . 1 . 37–43 . Jul 1995 . 7788527 . 10.1093/dnares/2.1.37 . free .
2. Charrasse S, Mazel M, Taviaux S, Berta P, Chow T, Larroque C . Characterization of the cDNA and pattern of expression of a new gene over-expressed in human hepatomas and colonic tumors . Eur. J. Biochem. . 234 . 2 . 406–13 . Feb 1996 . 8536682 . 10.1111/j.1432-1033.1995.406_b.x . free .
3. Web site: Entrez Gene: CKAP5 cytoskeleton associated protein 5.
4. Cassimeris L, Morabito J . TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly . Mol. Biol. Cell . 15 . 4 . 1580–90 . 2004 . 14718566 . 379257 . 10.1091/mbc.E03-07-0544 .
5. Barr AR, Gergely F . MCAK-independent functions of ch-Tog/XMAP215 in microtubule plus-end dynamics . Mol. Cell. Biol. . 28 . 23 . 7199–211 . 2008 . 18809577 . 2593372 . 10.1128/MCB.01040-08 .
6. Conte N, Delaval B, Ginestier C, Ferrand A, Isnardon D, Larroque C, Prigent C, Séraphin B, Jacquemier J, Birnbaum D . TACC1-chTOG-Aurora A protein complex in breast cancer . Oncogene . 22 . 50 . 8102–16 . Nov 2003 . 14603251 . 10.1038/sj.onc.1206972 . 10536264 .
7. Lauffart B, Howell SJ, Tasch JE, Cowell JK, Still IH . Interaction of the transforming acidic coiled-coil 1 (TACC1) protein with ch-TOG and GAS41/NuBI1 suggests multiple TACC1-containing protein complexes in human cells . Biochem. J. . 363 . Pt 1 . 195–200 . Apr 2002 . 11903063 . 1222467 . 10.1042/0264-6021:3630195 .