CILP explained

Cartilage intermediate layer protein 1 is a protein that in humans is encoded by the CILP gene.^{[1] [2] [3]}

Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. The synthesis of cartilage intermediate layer protein (CILP), which was identified and purified from human articular cartilage, increases in early osteoarthrosis cartilage. The C-terminal 460 amino acids of the protein show 90% similarity to the pig ectonucleotide pyrophosphohydrolase NTPPHase; this region is preceded by a furin protease consensus cleavage site. Thus, the CILP gene is thought to encode a protein precursor for 2 different proteins, namely CILP and a homolog of NTPPHase.

Further reading

• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 . etal.
• Nakamura I, Okawa A, Ikegawa S . Genomic organization, mapping, and polymorphisms of the gene encoding human cartilage intermediate layer protein (CILP) . J. Hum. Genet. . 44 . 3 . 203–5 . 1999 . 10319588 . 10.1007/s100380050143 . etal. free .
• Lorenzo P, Aman P, Sommarin Y, Heinegård D . The human CILP gene: exon/intron organization and chromosomal mapping . Matrix Biol. . 18 . 5 . 445–54 . 2000 . 10601732 . 10.1016/S0945-053X(99)00035-9 .
• Tsuruha J, Masuko-Hongo K, Kato T . Implication of cartilage intermediate layer protein in cartilage destruction in subsets of patients with osteoarthritis and rheumatoid arthritis . Arthritis Rheum. . 44 . 4 . 838–45 . 2001 . 11315923 . 10.1002/1529-0131(200104)44:4<838::AID-ANR140>3.0.CO;2-C . etal. free .
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Hirose J, Ryan LM, Masuda I . Up-regulated expression of cartilage intermediate-layer protein and ANK in articular hyaline cartilage from patients with calcium pyrophosphate dihydrate crystal deposition disease . Arthritis Rheum. . 46 . 12 . 3218–29 . 2003 . 12483726 . 10.1002/art.10632 . free .
• Johnson K, Farley D, Hu SI, Terkeltaub R . One of two chondrocyte-expressed isoforms of cartilage intermediate-layer protein functions as an insulin-like growth factor 1 antagonist . Arthritis Rheum. . 48 . 5 . 1302–14 . 2003 . 12746903 . 10.1002/art.10927 . free .
• Clark HF, Gurney AL, Abaya E . The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment . Genome Res. . 13 . 10 . 2265–70 . 2003 . 12975309 . 10.1101/gr.1293003 . 403697 . etal.
• Yao Z, Nakamura H, Masuko-Hongo K . Characterisation of cartilage intermediate layer protein (CILP)-induced arthropathy in mice . Ann. Rheum. Dis. . 63 . 3 . 252–8 . 2004 . 14962958 . 10.1136/ard.2003.008045 . 1754905 . etal.
• Du H, Masuko-Hongo K, Nakamura H . The prevalence of autoantibodies against cartilage intermediate layer protein, YKL-39, osteopontin, and cyclic citrullinated peptide in patients with early-stage knee osteoarthritis: evidence of a variety of autoimmune processes . Rheumatol. Int. . 26 . 1 . 35–41 . 2006 . 15378262 . 10.1007/s00296-004-0497-2 . 7221083 . etal.
• Gerhard DS, Wagner L, Feingold EA . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Seki S, Kawaguchi Y, Chiba K . A functional SNP in CILP, encoding cartilage intermediate layer protein, is associated with susceptibility to lumbar disc disease . Nat. Genet. . 37 . 6 . 607–12 . 2005 . 15864306 . 10.1038/ng1557 . 27694412 . etal.
• Mori M, Nakajima M, Mikami Y . Transcriptional regulation of the cartilage intermediate layer protein (CILP) gene . Biochem. Biophys. Res. Commun. . 341 . 1 . 121–7 . 2006 . 16413503 . 10.1016/j.bbrc.2005.12.159 . etal.
• Virtanen IM, Song YQ, Cheung KM . Phenotypic and population differences in the association between CILP and lumbar disc disease . J. Med. Genet. . 44 . 4 . 285–8 . 2007 . 17220213 . 10.1136/jmg.2006.047076 . 2598035 . etal.

Notes and References

1. Lorenzo P, Neame P, Sommarin Y, Heinegard D . Cloning and deduced amino acid sequence of a novel cartilage protein (CILP) identifies a proform including a nucleotide pyrophosphohydrolase . J Biol Chem . 273 . 36 . 23469–75 . Oct 1998 . 9722584 . 10.1074/jbc.273.36.23469 . free .
2. Lorenzo P, Bayliss MT, Heinegard D . A novel cartilage protein (CILP) present in the mid-zone of human articular cartilage increases with age . J Biol Chem . 273 . 36 . 23463–8 . Oct 1998 . 9722583 . 10.1074/jbc.273.36.23463 . free .
3. Web site: Entrez Gene: CILP cartilage intermediate layer protein, nucleotide pyrophosphohydrolase.