Calcineurin B homologous protein 1 explained

Calcineurin B homologous protein 1 is a protein encoded in humans by the CHP1 gene (formerly CHP).^[1]

Function

The protein encoded by this gene is a phosphoprotein that binds to the sodium-hydrogen exchangers (NHEs). This protein serves as an essential cofactor which supports the physiological activity of NHE family members. It has protein sequence similarity to calcineurin B and it is also known to be an endogenous inhibitor of calcineurin activity.^[1]

Interactions

Calcineurin B homologous protein 1 has been shown to interact with

• sodium-hydrogen antiporter 1,^{[2] [3] [4]}
• sodium-hydrogen antiporter 3,^[2] and
• sodium-hydrogen anti porter 9.^[5]

Further reading

• Robertson NG, Khetarpal U, Gutiérrez-Espeleta GA, Bieber FR, Morton CC . Isolation of novel and known genes from a human fetal cochlear cDNA library using subtractive hybridization and differential screening . Genomics . 23 . 1 . 42–50 . Sep 1994 . 7829101 . 10.1006/geno.1994.1457 .
• Barroso MR, Bernd KK, DeWitt ND, Chang A, Mills K, Sztul ES . A novel Ca2+-binding protein, p22, is required for constitutive membrane traffic . The Journal of Biological Chemistry . 271 . 17 . 10183–7 . Apr 1996 . 8626580 . 10.1074/jbc.271.17.10183 . free .
• Lin X, Barber DL . A calcineurin homologous protein inhibits GTPase-stimulated Na-H exchange . Proceedings of the National Academy of Sciences of the United States of America . 93 . 22 . 12631–6 . Oct 1996 . 8901634 . 38044 . 10.1073/pnas.93.22.12631 . 1996PNAS...9312631L . free .
• Lin X, Sikkink RA, Rusnak F, Barber DL . Inhibition of calcineurin phosphatase activity by a calcineurin B homologous protein . The Journal of Biological Chemistry . 274 . 51 . 36125–31 . Dec 1999 . 10593895 . 10.1074/jbc.274.51.36125 . free .
• Pang T, Su X, Wakabayashi S, Shigekawa M . Calcineurin homologous protein as an essential cofactor for Na+/H+ exchangers . The Journal of Biological Chemistry . 276 . 20 . 17367–72 . May 2001 . 11350981 . 10.1074/jbc.M100296200 . free .
• Matsumoto M, Miyake Y, Nagita M, Inoue H, Shitakubo D, Takemoto K, Ohtsuka C, Murakami H, Nakamura N, Kanazawa H . A serine/threonine kinase which causes apoptosis-like cell death interacts with a calcineurin B-like protein capable of binding Na(+)/H(+) exchanger . Journal of Biochemistry . 130 . 2 . 217–25 . Aug 2001 . 11481038 . 10.1093/oxfordjournals.jbchem.a002975 .
• Nakamura N, Miyake Y, Matsushita M, Tanaka S, Inoue H, Kanazawa H . KIF1Bbeta2, capable of interacting with CHP, is localized to synaptic vesicles . Journal of Biochemistry . 132 . 3 . 483–91 . Sep 2002 . 12204119 . 10.1093/oxfordjournals.jbchem.a003246 .
• Pang T, Wakabayashi S, Shigekawa M . Expression of calcineurin B homologous protein 2 protects serum deprivation-induced cell death by serum-independent activation of Na+/H+ exchanger . The Journal of Biological Chemistry . 277 . 46 . 43771–7 . Nov 2002 . 12226101 . 10.1074/jbc.M208313200 . free .
• Inoue H, Nakamura Y, Nagita M, Takai T, Masuda M, Nakamura N, Kanazawa H . Calcineurin homologous protein isoform 2 (CHP2), Na+/H+ exchangers-binding protein, is expressed in intestinal epithelium . Biological & Pharmaceutical Bulletin . 26 . 2 . 148–55 . Feb 2003 . 12576672 . 10.1248/bpb.26.148 . free .
• Pang T, Hisamitsu T, Mori H, Shigekawa M, Wakabayashi S . Role of calcineurin B homologous protein in pH regulation by the Na+/H+ exchanger 1: tightly bound Ca2+ ions as important structural elements . Biochemistry . 43 . 12 . 3628–36 . Mar 2004 . 15035633 . 10.1021/bi0360004 .
• Andrade J, Pearce ST, Zhao H, Barroso M . Interactions among p22, glyceraldehyde-3-phosphate dehydrogenase and microtubules . The Biochemical Journal . 384 . Pt 2 . 327–36 . Dec 2004 . 15312048 . 1134116 . 10.1042/BJ20040622 .
• Ben Ammar Y, Takeda S, Sugawara M, Miyano M, Mori H, Wakabayashi S . Crystallization and preliminary crystallographic analysis of the human calcineurin homologous protein CHP2 bound to the cytoplasmic region of the Na+/H+ exchanger NHE1 . Acta Crystallographica Section F . 61 . Pt 10 . 956–8 . Oct 2005 . 16511206 . 1991313 . 10.1107/S1744309105030836 .
• Mishima M, Wakabayashi S, Kojima C . Solution structure of the cytoplasmic region of Na+/H+ exchanger 1 complexed with essential cofactor calcineurin B homologous protein 1 . The Journal of Biological Chemistry . 282 . 4 . 2741–51 . Jan 2007 . 17050540 . 10.1074/jbc.M604092200 . free .

Notes and References

1. Web site: Entrez Gene: CHP calcium binding protein P22.
2. Inoue H, Nakamura Y, Nagita M, Takai T, Masuda M, Nakamura N, Kanazawa H . Calcineurin homologous protein isoform 2 (CHP2), Na+/H+ exchangers-binding protein, is expressed in intestinal epithelium . Biological & Pharmaceutical Bulletin . 26 . 2 . 148–55 . Feb 2003 . 12576672 . 10.1248/bpb.26.148 . free .
3. Lin X, Barber DL . A calcineurin homologous protein inhibits GTPase-stimulated Na-H exchange . Proceedings of the National Academy of Sciences of the United States of America . 93 . 22 . 12631–6 . Oct 1996 . 8901634 . 38044 . 10.1073/pnas.93.22.12631 . 1996PNAS...9312631L . free .
4. Pang T, Su X, Wakabayashi S, Shigekawa M . Calcineurin homologous protein as an essential cofactor for Na+/H+ exchangers . The Journal of Biological Chemistry . 276 . 20 . 17367–72 . May 2001 . 11350981 . 10.1074/jbc.M100296200 . free .
5. Zhang-James Y, DasBanerjee T, Sagvolden T, Middleton FA, Faraone SV . SLC9A9 mutations, gene expression, and protein-protein interactions in rat models of attention-deficit/hyperactivity disorder . American Journal of Medical Genetics Part B . 156B . 7 . 835–43 . Dec 2011 . 21858920 . 10.1002/ajmg.b.31229 . 3168688.