CEP350 explained

Centrosome-associated protein 350 is a protein that in humans is encoded by the CEP350 gene.^{[1] [2] [3]}

CEP350 is a large protein with a CAP-Gly domain typically found in cytoskeleton-associated proteins. It primarily localizes to the centrosome, a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. CEP350 is required to anchor microtubules at the centrosome. Furthermore, it increases the stability of growing centrioles.^[4]

It is also implicated in the regulation of a class of nuclear hormone receptors in the nucleus. Several alternatively spliced transcript variants have been found, but their full-length nature has not been determined.

Further reading

• Andersson B, Wentland MA, Ricafrente JY . A "double adaptor" method for improved shotgun library construction . Anal. Biochem. . 236 . 1 . 107–13 . 1996 . 8619474 . 10.1006/abio.1996.0138 . etal.
• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery . Genome Res. . 6 . 9 . 791–806 . 1997 . 8889548 . 10.1101/gr.6.9.791 . free .
• Yu W, Andersson B, Worley KC . Large-Scale Concatenation cDNA Sequencing . Genome Res. . 7 . 4 . 353–8 . 1997 . 9110174 . 10.1101/gr.7.4.353. 139146 . etal.
• Seki N, Ohira M, Nagase T . Characterization of cDNA clones in size-fractionated cDNA libraries from human brain . DNA Res. . 4 . 5 . 345–9 . 1998 . 9455484 . 10.1093/dnares/4.5.345 . etal. free .
• Makalowska I, Sood R, Faruque MU . Identification of six novel genes by experimental validation of GeneMachine predicted genes . Gene . 284 . 1–2 . 203–13 . 2002 . 11891061 . 10.1016/S0378-1119(01)00897-6 . etal.
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Andersen JS, Wilkinson CJ, Mayor T . Proteomic characterization of the human centrosome by protein correlation profiling . Nature . 426 . 6966 . 570–4 . 2003 . 14654843 . 10.1038/nature02166 . 2003Natur.426..570A . 4427303 . etal.
• Ota T, Suzuki Y, Nishikawa T . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . etal. free .
• Oh JH, Yang JO, Hahn Y . Transcriptome analysis of human gastric cancer . Mamm. Genome . 16 . 12 . 942–54 . 2006 . 16341674 . 10.1007/s00335-005-0075-2 . 69278 . etal.
• Kimura K, Wakamatsu A, Suzuki Y . Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes . Genome Res. . 16 . 1 . 55–65 . 2006 . 16344560 . 10.1101/gr.4039406 . 1356129 . etal.
• Nousiainen M, Silljé HH, Sauer G . Phosphoproteome analysis of the human mitotic spindle . Proc. Natl. Acad. Sci. U.S.A. . 103 . 14 . 5391–6 . 2006 . 16565220 . 10.1073/pnas.0507066103 . 1459365 . 2006PNAS..103.5391N . etal. free .
• Gregory SG, Barlow KF, McLay KE . The DNA sequence and biological annotation of human chromosome 1 . Nature . 441 . 7091 . 315–21 . 2006 . 16710414 . 10.1038/nature04727 . 2006Natur.441..315G . etal. free .
• Lim J, Hao T, Shaw C . A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration . Cell . 125 . 4 . 801–14 . 2006 . 16713569 . 10.1016/j.cell.2006.03.032 . 13709685 . etal. free .
• Olsen JV, Blagoev B, Gnad F . Global, in vivo, and site-specific phosphorylation dynamics in signaling networks . Cell . 127 . 3 . 635–48 . 2006 . 17081983 . 10.1016/j.cell.2006.09.026 . 7827573 . etal. free .
• Hoppeler-Lebel A, Celati C, Bellett G . Centrosomal CAP350 protein stabilises microtubules associated with the Golgi complex . J. Cell Sci. . 120 . Pt 18 . 3299–308 . 2007 . 17878239 . 10.1242/jcs.013102 . etal. free .

Notes and References

1. Yan X, Habedanck R, Nigg EA . A Complex of Two Centrosomal Proteins, CAP350 and FOP, Cooperates with EB1 in Microtubule Anchoring . Mol Biol Cell . 17 . 2 . 634–44 . Jan 2006 . 16314388 . 1356575 . 10.1091/mbc.E05-08-0810 .
2. Patel H, Truant R, Rachubinski RA, Capone JP . Activity and subcellular compartmentalization of peroxisome proliferator-activated receptor alpha are altered by the centrosome-associated protein CAP350 . J Cell Sci . 118 . Pt 1 . 175–86 . Dec 2004 . 15615782 . 10.1242/jcs.01600 . free .
3. Web site: Entrez Gene: CEP350 centrosomal protein 350kDa.
4. Le Clech. M. Role of CAP350 in centriolar tubule stability and centriole assembly.. PLOS ONE. 2008. 3. 12. e3855. 19052644. 10.1371/journal.pone.0003855. 2586089. 2008PLoSO...3.3855L. free.