CEP250 explained

Centrosome-associated protein CEP250 is a protein that in humans is encoded by the CEP250 gene.^{[1] [2] [3]} This gene encodes a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. The encoded protein dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. Furthermore, CEP135 is also required for the centriolar localization of CEP250.^[4]

Further reading

• Gerhard DS, Wagner L, Feingold EA . The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Jin J, Smith FD, Stark C . Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization . Curr. Biol. . 14 . 16 . 1436–50 . 2004 . 15324660 . 10.1016/j.cub.2004.07.051 . 2371325 . etal. free . 2004CBio...14.1436J .
• Colland F, Jacq X, Trouplin V . Functional Proteomics Mapping of a Human Signaling Pathway . Genome Res. . 14 . 7 . 1324–32 . 2004 . 15231748 . 10.1101/gr.2334104 . 442148 . etal.
• Faragher AJ, Fry AM . Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles . Mol. Biol. Cell . 14 . 7 . 2876–89 . 2004 . 12857871 . 10.1091/mbc.E03-02-0108 . 165683 .
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Mayor T, Hacker U, Stierhof YD, Nigg EA . The mechanism regulating the dissociation of the centrosomal protein C-Nap1 from mitotic spindle poles . J. Cell Sci. . 115 . Pt 16 . 3275–84 . 2003 . 10.1242/jcs.115.16.3275 . 12140259 .
• Deloukas P, Matthews LH, Ashurst J . The DNA sequence and comparative analysis of human chromosome 20 . Nature . 414 . 6866 . 865–71 . 2002 . 11780052 . 10.1038/414865a . 2001Natur.414..865D . etal. free .
• Mayor T, Stierhof YD, Tanaka K . The Centrosomal Protein C-Nap1 Is Required for Cell Cycle–Regulated Centrosome Cohesion . J. Cell Biol. . 151 . 4 . 837–46 . 2000 . 11076968 . 10.1083/jcb.151.4.837 . 2169446 . etal.
• Hirsch DS, Pirone DM, Burbelo PD . A new family of Cdc42 effector proteins, CEPs, function in fibroblast and epithelial cell shape changes . J. Biol. Chem. . 276 . 2 . 875–83 . 2001 . 11035016 . 10.1074/jbc.M007039200 . free .
• Helps NR, Luo X, Barker HM, Cohen PT . NIMA-related kinase 2 (Nek2), a cell-cycle-regulated protein kinase localized to centrosomes, is complexed to protein phosphatase 1 . Biochem. J. . 349 . Pt 2 . 509–18 . 2001 . 10880350 . 10.1042/0264-6021:3490509 . 1221174 .

Notes and References

1. Mack GJ, Rees J, Sandblom O, Balczon R, Fritzler MJ, Rattner JB . Autoantibodies to a group of centrosomal proteins in human autoimmune sera reactive with the centrosome . Arthritis Rheum . 41 . 3 . 551–8 . Apr 1998 . 9506584 . 10.1002/1529-0131(199803)41:3<551::AID-ART22>3.0.CO;2-X . free .
2. Fry AM, Mayor T, Meraldi P, Stierhof YD, Tanaka K, Nigg EA . C-Nap1, a Novel Centrosomal Coiled-Coil Protein and Candidate Substrate of the Cell Cycle–regulated Protein Kinase Nek2 . J Cell Biol . 141 . 7 . 1563–74 . Aug 1998 . 9647649 . 2133000 . 10.1083/jcb.141.7.1563 .
3. Web site: Entrez Gene: CEP250 centrosomal protein 250kDa.
4. Kim K, Lee S, Chang J, Rhee K . A novel function of CEP135 as a platform protein of C-NAP1 for its centriolar localization . Exp. Cell Res. . 314 . 20 . 3692–700 . December 2008 . 18851962 . 10.1016/j.yexcr.2008.09.016 .