CDK5RAP1 explained

CDK5 regulatory subunit-associated protein 1 is a protein that in humans is encoded by the CDK5RAP1 gene.^{[1] [2] [3] [4]}

Neuronal CDC2-like kinase, which is involved in the regulation of neuronal differentiation, is composed of a catalytic subunit, CDK5, and an activating subunit, p25NCK5A. The protein encoded by this gene binds to p25NCK5A and therefore may be involved in neuronal differentiation. Multiple transcript variants exist for this gene, but the full-length natures of only two have been determined.^[4]

Further reading

• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K . Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library . Gene . 200 . 1–2 . 149–56 . 1997 . 9373149 . 10.1016/S0378-1119(97)00411-3 . etal.
• Lai CH, Chou CY, Ch'ang LY . Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics . Genome Res. . 10 . 5 . 703–13 . 2000 . 10810093 . 10.1101/gr.10.5.703 . 310876 . etal.
• Wang X, Ching YP, Lam WH . Identification of a common protein association region in the neuronal Cdk5 activator . J. Biol. Chem. . 275 . 41 . 31763–9 . 2000 . 10915792 . 10.1074/jbc.M004358200 . etal. free .
• Zhang QH, Ye M, Wu XY . Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells . Genome Res. . 10 . 10 . 1546–60 . 2001 . 11042152 . 10.1101/gr.140200 . 310934 . etal.
• Deloukas P, Matthews LH, Ashurst J . The DNA sequence and comparative analysis of human chromosome 20 . Nature . 414 . 6866 . 865–71 . 2002 . 11780052 . 10.1038/414865a . 2001Natur.414..865D . etal. free .
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Ota T, Suzuki Y, Nishikawa T . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . etal. free .
• Lehner B, Sanderson CM . A protein interaction framework for human mRNA degradation . Genome Res. . 14 . 7 . 1315–23 . 2004 . 15231747 . 10.1101/gr.2122004 . 442147 .
• Gerhard DS, Wagner L, Feingold EA . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Rual JF, Venkatesan K, Hao T . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 . etal.

Notes and References

1. Ching YP, Qi Z, Wang JH . Cloning of three novel neuronal Cdk5 activator binding proteins . Gene . 242 . 1–2 . 285–94 . Apr 2000 . 10721722 . 10.1016/S0378-1119(99)00499-0 .
2. Ching YP, Pang AS, Lam WH, Qi RZ, Wang JH . Identification of a neuronal Cdk5 activator-binding protein as Cdk5 inhibitor . J Biol Chem . 277 . 18 . 15237–40 . Apr 2002 . 11882646 . 10.1074/jbc.C200032200 . free .
3. Zou X, Ji C, Jin F, Liu J, Wu M, Zheng H, Wang Y, Li X, Xu J, Gu S, Xie Y, Mao Y . Cloning, characterization and expression of CDK5RAP1_v3 and CDK5RAP1_v4, two novel splice variants of human CDK5RAP1 . Genes Genet Syst . 79 . 3 . 177–82 . Aug 2004 . 15329498 . 10.1266/ggs.79.177 . free .
4. Web site: Entrez Gene: CDK5RAP1 CDK5 regulatory subunit associated protein 1.