CDC5L explained

Cell division cycle 5-like protein is a protein that in humans is encoded by the CDC5L gene.^{[1] [2] [3]}

Function

The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G₂/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G₂/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing.^[3]

Interactions

CDC5L has been shown to interact with:

• ASF/SF2,
• BZW1,
• CWC15,
• DNA-PKcs,
• DYNC1H1,
• GCN1L1,
• HSPA8,
• ILF2,
• PLRG1,^{[4] [5] [6]}
• PPM1D,
• PPP1CA,
• PRPF19,
• RBMX and
• RPL12,
• RPL13,
• RPS16,
• RPS25,
• SF3A1,
• SF3B1,
• SF3B2,
• SF3B4,
• SFPQ,
• SRSF2,
• SNRPA1,
• SNRPD3,
• SRRM1,^[7]
• Small nuclear ribonucleoprotein D1,^[7]
• Small nuclear ribonucleoprotein D2,^[7]
• Small nuclear ribonucleoprotein polypeptide A,^[7]
• TOP2A,^[7] and
• TTF2.^[8]

See also

• G2/M checkpoint

Further reading

• Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T . Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones . DNA Research . 9 . 3 . 99–106 . Jun 2002 . 12168954 . 10.1093/dnares/9.3.99 . 10.1.1.500.923 .
• Heaney JP, Jacobson A . Simplified control of upper abdominal hemorrhage from the vena cava . Surgery . 78 . 2 . 138–41 . Aug 1975 . 1154257 .
• Hirayama T, Shinozaki K . A cdc5+ homolog of a higher plant, Arabidopsis thaliana . Proceedings of the National Academy of Sciences of the United States of America . 93 . 23 . 13371–6 . Nov 1996 . 8917598 . 24100 . 10.1073/pnas.93.23.13371 . free .
• Ishikawa K, Nagase T, Nakajima D, Seki N, Ohira M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O . Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro . DNA Research . 4 . 5 . 307–13 . Oct 1997 . 9455477 . 10.1093/dnares/4.5.307 . free .
• Bernstein HS, Coughlin SR . A mammalian homolog of fission yeast Cdc5 regulates G2 progression and mitotic entry . The Journal of Biological Chemistry . 273 . 8 . 4666–71 . Feb 1998 . 9468527 . 10.1074/jbc.273.8.4666 . free .
• Joseph S. Lipsick . Ohi R, Feoktistova A, McCann S, Valentine V, Look AT, Lipsick JS, Gould KL . Myb-related Schizosaccharomyces pombe cdc5p is structurally and functionally conserved in eukaryotes . Molecular and Cellular Biology . 18 . 7 . 4097–108 . Jul 1998 . 9632794 . 108994 . 10.1128/mcb.18.7.4097.
• Neubauer G, King A, Rappsilber J, Calvio C, Watson M, Ajuh P, Sleeman J, Lamond A, Mann M . Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex . Nature Genetics . 20 . 1 . 46–50 . Sep 1998 . 9731529 . 10.1038/1700 . 585778 .
• Burns CG, Ohi R, Krainer AR, Gould KL . Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing . Proceedings of the National Academy of Sciences of the United States of America . 96 . 24 . 13789–94 . Nov 1999 . 10570151 . 24143 . 10.1073/pnas.96.24.13789 . free . 1999PNAS...9613789B .
• Boudrez A, Beullens M, Groenen P, Van Eynde A, Vulsteke V, Jagiello I, Murray M, Krainer AR, Stalmans W, Bollen M . NIPP1-mediated interaction of protein phosphatase-1 with CDC5L, a regulator of pre-mRNA splicing and mitotic entry . The Journal of Biological Chemistry . 275 . 33 . 25411–7 . Aug 2000 . 10827081 . 10.1074/jbc.M001676200 . free .
• Lei XH, Shen X, Xu XQ, Bernstein HS . Human Cdc5, a regulator of mitotic entry, can act as a site-specific DNA binding protein . Journal of Cell Science . 113 . 24. 4523–31 . Dec 2000 . 11082045 . 113 . 10.1242/jcs.113.24.4523 .
• Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI . Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry . The EMBO Journal . 19 . 23 . 6569–81 . Dec 2000 . 11101529 . 305846 . 10.1093/emboj/19.23.6569 .
• Harrington JJ, Sherf B, Rundlett S, Jackson PD, Perry R, Cain S, Leventhal C, Thornton M, Ramachandran R, Whittington J, Lerner L, Costanzo D, McElligott K, Boozer S, Mays R, Smith E, Veloso N, Klika A, Hess J, Cothren K, Lo K, Offenbacher J, Danzig J, Ducar M . Creation of genome-wide protein expression libraries using random activation of gene expression . Nature Biotechnology . 19 . 5 . 440–5 . May 2001 . 11329013 . 10.1038/88107 . 25064683 .
• Ajuh P, Sleeman J, Chusainow J, Lamond AI . A direct interaction between the carboxyl-terminal region of CDC5L and the WD40 domain of PLRG1 is essential for pre-mRNA splicing . The Journal of Biological Chemistry . 276 . 45 . 42370–81 . Nov 2001 . 11544257 . 10.1074/jbc.M105453200 . free .
• Jurica MS, Licklider LJ, Gygi SR, Grigorieff N, Moore MJ . Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis . RNA . 8 . 4 . 426–39 . Apr 2002 . 11991638 . 1370266 . 10.1017/S1355838202021088 .
• Leonard D, Ajuh P, Lamond AI, Legerski RJ . hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing . Biochemical and Biophysical Research Communications . 308 . 4 . 793–801 . Sep 2003 . 12927788 . 10.1016/S0006-291X(03)01486-4 . 10.1.1.539.8359 .
• Liu L, Gräub R, Hlaing M, Epting CL, Turck CW, Xu XQ, Zhang L, Bernstein HS . Distinct domains of human CDC5 direct its nuclear import and association with the spliceosome . . 39 . 2 . 119–32 . 2004 . 14515018 . 10.1385/CBB:39:2:119 . 22472390 .

Notes and References

1. Groenen PM, Vanderlinden G, Devriendt K, Fryns JP, Van de Ven WJ . Rearrangement of the human CDC5L gene by a t(6;19)(p21;q13.1) in a patient with multicystic renal dysplasia . Genomics . 49 . 2 . 218–29 . Apr 1998 . 9598309 . 10.1006/geno.1998.5254 .
2. Bernstein HS, Coughlin SR . Pombe Cdc5-related protein. A putative human transcription factor implicated in mitogen-activated signaling . The Journal of Biological Chemistry . 272 . 9 . 5833–7 . Feb 1997 . 9038199 . 10.1074/jbc.272.9.5833 . free .
3. Web site: Entrez Gene: CDC5L CDC5 cell division cycle 5-like (S. pombe).
4. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D . Large-scale mapping of human protein-protein interactions by mass spectrometry . Molecular Systems Biology . 3 . 89 . 17353931 . 1847948 . 10.1038/msb4100134 . Bukhman Yury V . Ethier Martin . Zhang Shudong . Ornatsky Olga . Heilbut Adrian . Moore Lynda . Morin Gregg B . Ho Yuen . Figeys Daniel . Muskat Brenda . Kinach Robert . Moran Michael F . Adams Sally-Lin . Gladwish Katharine . Colwill Karen . Lambert Jean-Philippe . Abu-Farha Mohamed . Vasilescu Julian . Sheng Yinglun . Hogue Kelly . Kuehl Bonnie . Stewart Ian I . Duewel Henry S . Topaloglou Thodoros . 2007.
5. Ajuh P, Lamond AI . Identification of peptide inhibitors of pre-mRNA splicing derived from the essential interaction domains of CDC5L and PLRG1 . Nucleic Acids Research . 31 . 21 . 6104–16 . Nov 2003 . 14576297 . 275459 . 10.1093/nar/gkg817.
6. Ajuh P, Sleeman J, Chusainow J, Lamond AI . A direct interaction between the carboxyl-terminal region of CDC5L and the WD40 domain of PLRG1 is essential for pre-mRNA splicing . The Journal of Biological Chemistry . 276 . 45 . 42370–81 . Nov 2001 . 11544257 . 10.1074/jbc.M105453200 . free .
7. Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI . Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry . The EMBO Journal . 19 . 23 . 6569–81 . Dec 2000 . 11101529 . 305846 . 10.1093/emboj/19.23.6569 .
8. Leonard D, Ajuh P, Lamond AI, Legerski RJ . hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing . Biochemical and Biophysical Research Communications . 308 . 4 . 793–801 . Sep 2003 . 12927788 . 10.1016/s0006-291x(03)01486-4. 10.1.1.539.8359 .