CDC42EP3 explained

Cdc42 effector protein 3 is a protein that in humans is encoded by the CDC42EP3 gene.^{[1] [2] [3]}

CDC42, a small Rho GTPase, regulates the formation of F-actin-containing structures through its interaction with the downstream effector proteins. The protein encoded by this gene is a member of the Borg family of CDC42 effector proteins. Borg family proteins contain a CRIB (Cdc42/Rac interactive-binding) domain. They bind to, and negatively regulate the function of, CDC42. This protein can interact with CDC42, as well as with the ras homolog gene family, member Q (ARHQ/TC10). Expression of this protein in fibroblasts has been shown to induce pseudopodia formation.

Interactions

CDC42EP3 has been shown to interact with CDC42^[4] and RHOQ.^[4]

Further reading

• Joberty G, Perlungher RR, Macara IG . The Borgs, a new family of Cdc42 and TC10 GTPase-interacting proteins . Mol. Cell. Biol. . 19 . 10 . 6585–97 . 2000 . 10490598 . 10.1128/MCB.19.10.6585. 84628 .
• Hartley JL, Temple GF, Brasch MA . DNA cloning using in vitro site-specific recombination . Genome Res. . 10 . 11 . 1788–95 . 2001 . 11076863 . 10.1101/gr.143000 . 310948 .
• Wiemann S, Weil B, Wellenreuther R . Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs . Genome Res. . 11 . 3 . 422–35 . 2001 . 11230166 . 10.1101/gr.GR1547R . 311072 . etal.
• Simpson JC, Wellenreuther R, Poustka A . Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing . EMBO Rep. . 1 . 3 . 287–92 . 2001 . 11256614 . 10.1093/embo-reports/kvd058 . 1083732 . etal.
• Joberty G, Perlungher RR, Sheffield PJ . Borg proteins control septin organization and are negatively regulated by Cdc42 . Nat. Cell Biol. . 3 . 10 . 861–6 . 2001 . 11584266 . 10.1038/ncb1001-861 . 58805 . etal.
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . etal. 2002PNAS...9916899M . free .
• Ota T, Suzuki Y, Nishikawa T . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . etal. free .
• Ballif BA, Villén J, Beausoleil SA . Phosphoproteomic analysis of the developing mouse brain . Mol. Cell. Proteomics . 3 . 11 . 1093–101 . 2005 . 15345747 . 10.1074/mcp.M400085-MCP200 . etal. free .
• Gerhard DS, Wagner L, Feingold EA . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Wiemann S, Arlt D, Huber W . From ORFeome to biology: a functional genomics pipeline . Genome Res. . 14 . 10B . 2136–44 . 2004 . 15489336 . 10.1101/gr.2576704 . 528930 . etal.
• Hillier LW, Graves TA, Fulton RS . Generation and annotation of the DNA sequences of human chromosomes 2 and 4 . Nature . 434 . 7034 . 724–31 . 2005 . 15815621 . 10.1038/nature03466 . etal. 2005Natur.434..724H . free .
• Stelzl U, Worm U, Lalowski M . A human protein-protein interaction network: a resource for annotating the proteome . Cell . 122 . 6 . 957–68 . 2005 . 16169070 . 10.1016/j.cell.2005.08.029 . etal. 11858/00-001M-0000-0010-8592-0 . 8235923 . free .
• Rual JF, Venkatesan K, Hao T . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . etal. 2005Natur.437.1173R . 4427026 .
• Mehrle A, Rosenfelder H, Schupp I . The LIFEdb database in 2006 . Nucleic Acids Res. . 34 . Database issue . D415–8 . 2006 . 16381901 . 10.1093/nar/gkj139 . 1347501 . etal.
• Olsen JV, Blagoev B, Gnad F . Global, in vivo, and site-specific phosphorylation dynamics in signaling networks . Cell . 127 . 3 . 635–48 . 2006 . 17081983 . 10.1016/j.cell.2006.09.026 . 7827573 . etal. free .

Notes and References

1. Alberts AS, Bouquin N, Johnston LH, Treisman R . Analysis of RhoA-binding proteins reveals an interaction domain conserved in heterotrimeric G protein beta subunits and the yeast response regulator protein Skn7 . J Biol Chem . 273 . 15 . 8616–22 . May 1998 . 9535835 . 10.1074/jbc.273.15.8616 . free .
2. Hirsch DS, Pirone DM, Burbelo PD . A new family of Cdc42 effector proteins, CEPs, function in fibroblast and epithelial cell shape changes . J Biol Chem . 276 . 2 . 875–83 . Mar 2001 . 11035016 . 10.1074/jbc.M007039200 . free .
3. Web site: Entrez Gene: CDC42EP3 CDC42 effector protein (Rho GTPase binding) 3.
4. Joberty . G . Perlungher R R. Macara I G . Oct 1999 . The Borgs, a new family of Cdc42 and TC10 GTPase-interacting proteins . Mol. Cell. Biol. . 19 . 10 . 6585–97 . 0270-7306. 10490598 . 84628 . 10.1128/MCB.19.10.6585 .