CCDC186 is a protein that in humans is encoded by the CCDC186 gene[1] The CCDC186 gene is also known as the CTCL-tumor associated antigen with accession number NM_018017.[2]
CCDC186 has the chromosome location of 10q25.3 and is 53,750 bases in size oriented on the minus strand. PSORTII Protein k-NN Prediction indicated that C10orf118 is 65.2% of the time nuclear, 17.4% cytosolic, 8.7% mitochondrial, 4.3% vesicles of secretory system, and 4.3% endoplasmic reticulum.[3]
Analysis of gene expression in humans and other species indicates C10orf118 is ubiquitously expressed in all tissue types at varying developmental stages. An EST profile from NCBI displayed the greatest expression in bone marrow, kidneys, and the prostate cell lines. Breakdown by health state indicates high expression of C10orf118 in bladder carcinoma and prostate cancer.[4]
The protein of CCDC186 (NP_060487) is 898 amino acids in length. The predicted molecular weight is 103.7kdal and the isoelectric point is predicted to be 5.92.[5]
A serine rich region is observed in amino acids 710-747. A compositional analysis revealed that C10orf118 is Proline (1.1%) poor and Glutamic acid (14.1%) and Lysine (12.0%) rich.
CCDC186 protein was found to interact with proteins PLEKAH5, Ezra, GAMMAHV.ORF23, and SMAD3.[6] [7]
Orthologous sequences of CCDC186 were not found to be in bacteria, archea, protist, or plants. CCDC186 has no human paralogs. The date of divergence for the orthologous sequences highly correlates with the sequences similarity in that the percent identity decreases as you go back in time. Closely related orthologs include mammals and birds and moderately related orthologs include other vertebrates such as fish, reptiles, and amphibians. Distantly related orthologous sequences are primarily observed in invertebrates.[8]
| Sequence Number | Genus and species | Common name | Date of divergence (MYA) | Accession number | Sequence length | Sequence identity | Sequence similarity | E-value | |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Homo sapiens | C10orf118 | 0 | NM_018017 | 898 aa | 100% | 100% | 0% | |
| 2 | Nomascus leucogenys | Northern White-cheeked Gibbon | 19.9 | XP_003255542 | 898 aa | 98% | 98% | 0% | |
| 3 | Chinchilla lanigera | long tailed chinchilla | 90.9 | XP_013371303 | 906 aa | 90% | 94% | 0% | |
| 4 | Mus musculus | house mouse | 90.9 | NP_739563 | 917 aa | 84% | 91% | 0% | |
| 5 | Tursiops truncatus | bottlense dolphin | 97.5 | XP_004318425 | 599 aa | 60% | 68% | 0% | |
| 6 | Loxodonta africana | African Bush Elephant | 105 | XP_010596062 | 805 aa | 90% | 91% | 0% | |
| 7 | Egretta garzetta | little egret | 320.5 | XP_009634185 | 914 aa | 75% | 83% | 0% | |
| 9 | Chelonia mydas | geen sea turtle | 320.5 | XP_007056391 | 920 aa | 76% | 83% | 0% | |
| 10 | Xenopus tropicalis | western clawed frog | 355.7 | XP_004919435 | 878 aa | 71% | 83% | 0% | |
| 11 | Astyanax mexicanus | Mexican tetra | 429.6 | XP_007259323 | 987 aa | 71% | 82% | 5.00E-09 | |
| 12 | Oryzias latipes | Japanese rice fish | 429.6 | XP_011486278 | 991 aa | 58% | 71% | 0% | |
| 13 | Callorhinchus milii | Australian ghostshark | 482.9 | XP_007903851 | 931 aa | 65% | 79% | 0% | |
| 14 | Strongylocentrotus purpuratus | Sea Urchin | 747.8 | XP_011675415 | 884 aa | 39% | 58% | 7.00E-84 | |
| 15 | Octopus bimaculoides | California Two-spot Octopus | 847 | XP_014782587 | 1221 aa | 42% | 60% | 3.00E-127 | |
| 16 | Aplysiomorpha | sea hare | 847 | Not found in BLAST | 1384 aa | 42% | 63% | 3.00E-145 | |
| 17 | Orussus abietinus | wasp | 847 | XP_012288999 | 1057 aa | 36% | 55% | 5.00E-100 | |
| 18 | Atta cephalotes | leafcutter ant | 847 | XP_012054311 | 1154 aa | 35% | 55% | 9.00E-93 | |
| 19 | Helobdella robusta | leech | 847 | ESO07814 | 997 aa | 29% | 47% | 1.00E-50 | |
| 20 | Caenorhabditis elegans | nematode | 847 | NP_871700 | 743 aa | 26% | 34% | 1.00E-25 | |
| 21 | Trichoplax adhaerens | NA | 936 | EDV25816 | 976 aa | 26% | 46% | 2.00E-11 |
| Motif Information[9] | Position(s) | |
|---|---|---|
| N-glycosylation site | 33-36, 40-43, 109-112, 867-870 | |
| cAMP- and cGMP-dependent protein kinase phosphorylation site. | 238-241, 583-586, 794-797 | |
| Casein kinase II phosphorylation site. | 2-5, 10-13, 31-34, 36-39, 126-129, 130-133, 135-138, 139-142, 157-160, 228-231, 241-244, 256-259, 405-408, 440-443, 459-462, 490-493, 515-518, 611-614, 617-620, 625-628, 671-674, 690-693, 734-737, 869-872, 879-882 | |
| Leucine Zipper Pattern | 588-609, 870-891 | |
| N-myristoylation site | 32-37, 75-80, 86-91, 183-188, 721-726, 728-733, 745-750, 810-815, 828-833 | |
| Protein kinase C phosphorylation site | 12-14, 35-37, 49-51, 146-148, 228-230, 243-245, 250-252, 278-280, 347-349, 371-373, 405-407, 417-419, 490-492, 500-502, 581-583, 592-594, 596-598, 682-684, 690-692, 825-827, 869-871, 895-897 | |
| Tyrosine kinase phosphorylation site | 134-141, 514-522 | |
| Cullen Family Profile | 443-552 | |
| K-Box Domain Profile | 526-619 | |
| Nebulin Repeat Profile | 521-531 | |
| Serine Rich Region | 710-747 | |
| Rad50 zinc-hook domain profile | 460-562 | |
| ATP synthase B/B' CF(0) | 405-433 | |
| Alpha-2-macroglobulin RAP, C-terminal domain | 524-705 | |
| 334-577 |
CCDC186 is predicted to undergo multiple posttranslational modifications including predicted O-beta-GlcNAc attachment, phosphorylation, a nuclear export signal, glycation of lysines, GlcNAc O-glycosylation, N-glycosylation, and NetCorona sites.[10]
Prior research indicates that the open reading frame of C10orf118 is linked to cutaneous T-cell lymphoma by a tumor antigen L14-2.[11] The protein CCDC186 is also found at higher than normal levels in the breast cancer cell line BC 8701.[12]