Macrophage-capping protein explained

Macrophage-capping protein (CAPG) also known as actin regulatory protein CAP-G is a protein that in humans is encoded by the CAPG gene.^{[1] [2] [3]}

Function

This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins.^[4] The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively, spliced transcript variants have been observed, but have not been fully described.

Further reading

• Southwick FS . Gain-of-function mutations conferring actin-severing activity to human macrophage cap G . J. Biol. Chem. . 270 . 1 . 45–8 . 1995 . 7814409 . 10.1074/jbc.270.1.45 . free .
• Mishra VS, Henske EP, Kwiatkowski DJ, Southwick FS . The human actin-regulatory protein cap G: gene structure and chromosome location . Genomics . 23 . 3 . 560–5 . 1995 . 7851883 . 10.1006/geno.1994.1543 .
• Maruyama K, Sugano S . Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides . Gene . 138 . 1–2 . 171–4 . 1994 . 8125298 . 10.1016/0378-1119(94)90802-8 .
• Hirano T, Kobayashi R, Hirano M . Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein . Cell . 89 . 4 . 511–21 . 1997 . 9160743 . 10.1016/S0092-8674(00)80233-0 . 15061740 . free .
• Cabello OA, Eliseeva E, He WG, etal . Cell cycle-dependent expression and nucleolar localization of hCAP-H . Mol. Biol. Cell . 12 . 11 . 3527–37 . 2002 . 11694586 . 10.1091/mbc.12.11.3527. 60273 .
• Van Impe K, De Corte V, Eichinger L, etal . The Nucleo-cytoplasmic actin-binding protein CapG lacks a nuclear export sequence present in structurally related proteins . J. Biol. Chem. . 278 . 20 . 17945–52 . 2003 . 12637565 . 10.1074/jbc.M209946200 . free .
• De Corte V, Van Impe K, Bruyneel E, etal . Increased importin-beta-dependent nuclear import of the actin modulating protein CapG promotes cell invasion . J. Cell Sci. . 117 . Pt 22 . 5283–92 . 2005 . 15454578 . 10.1242/jcs.01410 . free .
• Watari A, Takaki K, Higashiyama S, etal . Suppression of tumorigenicity, but not anchorage independence, of human cancer cells by new candidate tumor suppressor gene CapG . Oncogene . 25 . 56 . 7373–80 . 2007 . 16767159 . 10.1038/sj.onc.1209732 . free .
• Chi A, Valencia JC, Hu ZZ, etal . Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes . J. Proteome Res. . 5 . 11 . 3135–44 . 2007 . 17081065 . 10.1021/pr060363j .

Notes and References

1. Dabiri GA, Young CL, Rosenbloom J, Southwick FS . Molecular cloning of human macrophage capping protein cDNA. A unique member of the gelsolin/villin family expressed primarily in macrophages . J Biol Chem . 267 . 23 . 16545–52 . Sep 1992 . 10.1016/S0021-9258(18)42037-6 . 1322908 . free .
2. Pellieux C, Desgeorges A, Pigeon CH, Chambaz C, Yin H, Hayoz D, Silacci P . Cap G, a gelsolin family protein modulating protective effects of unidirectional shear stress . J Biol Chem . 278 . 31 . 29136–44 . Jul 2003 . 12754261 . 10.1074/jbc.M300598200 . free .
3. Web site: Entrez Gene: CAPG capping protein (actin filament), gelsolin-like.
4. 24155256. 2013. Ghoshdastider. U. The expanding superfamily of gelsolin homology domain proteins. Cytoskeleton. 70. 11. 775–95. Popp. D. Burtnick. L. D.. Robinson. R. C.. 10.1002/cm.21149. 205643538.