CADPS explained

Calcium-dependent secretion activator 1 is a protein that in humans is encoded by the CADPS gene.^{[1] [2]}

CADPS encodes a novel neural/endocrine-specific cytosolic and peripheral membrane protein required for the Ca2+-regulated exocytosis of secretory vesicles. CADPS acts at a stage in exocytosis that follows ATP-dependent priming, which involves the essential synthesis of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Alternative splicing has been observed at this locus and three variants, encoding distinct isoforms, are described.^[2]

Further reading

• Nakajima D, Okazaki N, Yamakawa H . Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones. . DNA Res. . 9 . 3 . 99–106 . 2003 . 12168954 . 10.1093/dnares/9.3.99 . etal. free .
• Nishizaki T, Walent JH, Kowalchyk JA, Martin TF . A key role for a 145-kDa cytosolic protein in the stimulation of Ca(2+)-dependent secretion by protein kinase C. . J. Biol. Chem. . 267 . 33 . 23972–81 . 1992 . 10.1016/S0021-9258(18)35932-5 . 1429734 . free .
• Ann K, Kowalchyk JA, Loyet KM, Martin TF . Novel Ca2+-binding protein (CAPS) related to UNC-31 required for Ca2+-activated exocytosis. . J. Biol. Chem. . 272 . 32 . 19637–40 . 1997 . 9289490 . 10.1074/jbc.272.32.19637 . free .
• Loyet KM, Kowalchyk JA, Chaudhary A . Specific binding of phosphatidylinositol 4,5-bisphosphate to calcium-dependent activator protein for secretion (CAPS), a potential phosphoinositide effector protein for regulated exocytosis. . J. Biol. Chem. . 273 . 14 . 8337–43 . 1998 . 9525942 . 10.1074/jbc.273.14.8337 . etal. free .
• Berwin B, Floor E, Martin TF . CAPS (mammalian UNC-31) protein localizes to membranes involved in dense-core vesicle exocytosis. . Neuron . 21 . 1 . 137–45 . 1998 . 9697858 . 10.1016/S0896-6273(00)80521-8 . free .
• Hirosawa M, Nagase T, Ishikawa K . Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain. . DNA Res. . 6 . 5 . 329–36 . 2000 . 10574461 . 10.1093/dnares/6.5.329 . etal. free .
• Nakayama M, Kikuno R, Ohara O . Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs. . Genome Res. . 12 . 11 . 1773–84 . 2003 . 12421765 . 10.1101/gr.406902 . 187542 .
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Cisternas FA, Vincent JB, Scherer SW, Ray PN . Cloning and characterization of human CADPS and CADPS2, new members of the Ca2+-dependent activator for secretion protein family. . Genomics . 81 . 3 . 279–91 . 2003 . 12659812 . 10.1016/S0888-7543(02)00040-X .
• Speidel D, Varoqueaux F, Enk C . A family of Ca2+-dependent activator proteins for secretion: comparative analysis of structure, expression, localization, and function. . J. Biol. Chem. . 278 . 52 . 52802–9 . 2004 . 14530279 . 10.1074/jbc.M304727200 . etal. free .
• Ota T, Suzuki Y, Nishikawa T . Complete sequencing and characterization of 21,243 full-length human cDNAs. . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . etal. free .
• Gerhard DS, Wagner L, Feingold EA . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Binda AV, Kabbani N, Levenson R . Regulation of dense core vesicle release from PC12 cells by interaction between the D2 dopamine receptor and calcium-dependent activator protein for secretion (CAPS). . Biochem. Pharmacol. . 69 . 10 . 1451–61 . 2005 . 15857609 . 10.1016/j.bcp.2005.02.015 .

Notes and References

1. Walent JH, Porter BW, Martin TF . A novel 145 kd brain cytosolic protein reconstitutes Ca(2+)-regulated secretion in permeable neuroendocrine cells . Cell . 70 . 5 . 765–75 . Oct 1992 . 1516133 . 10.1016/0092-8674(92)90310-9 . 29122392 .
2. Web site: Entrez Gene: CADPS Ca2+-dependent secretion activator.