GCFC2 explained

GC-rich sequence DNA-binding factor is a protein that in humans is encoded by the GCFC2 gene.^{[1] [2] [3] [4]}

The first mRNA transcript isolated for this gene was part of an artificial chimera derived from two distinct gene transcripts and a primer used in the cloning process (see Genbank accession M29204). A positively charged amino terminus present only in the chimera was determined to bind GC-rich DNA, thus mistakenly thought to identify a transcription factor gene.

Further reading

• Mao P . [Revisions of the cDNA and primary protein structure of human transcription factor GCF] . Hokkaido Igaku Zasshi . 74 . 4 . 315–30 . 1999 . 10480038 .
• Takimoto M, Mao P, Wei G . Molecular analysis of the GCF gene identifies revisions to the cDNA and amino acid sequences(1) . Biochim. Biophys. Acta . 1447 . 1 . 125–31 . 1999 . 10500253 . 10.1016/S0167-4781(99)00127-X. etal.
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Ota T, Suzuki Y, Nishikawa T . Complete sequencing and characterization of 21,243 full-length human cDNAs . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . etal. free .
• Beausoleil SA, Jedrychowski M, Schwartz D . Large-scale characterization of HeLa cell nuclear phosphoproteins . Proc. Natl. Acad. Sci. U.S.A. . 101 . 33 . 12130–5 . 2004 . 15302935 . 10.1073/pnas.0404720101 . 514446 . 2004PNAS..10112130B . etal. free .
• Gerhard DS, Wagner L, Feingold EA . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Hillier LW, Graves TA, Fulton RS . Generation and annotation of the DNA sequences of human chromosomes 2 and 4 . Nature . 434 . 7034 . 724–31 . 2005 . 15815621 . 10.1038/nature03466 . 2005Natur.434..724H . etal. free .
• Olsen JV, Blagoev B, Gnad F . Global, in vivo, and site-specific phosphorylation dynamics in signaling networks . Cell . 127 . 3 . 635–48 . 2006 . 17081983 . 10.1016/j.cell.2006.09.026 . 7827573 . etal. free .

Notes and References

1. Johnson AC, Kageyama R, Popescu NC, Pastan I . Expression and chromosomal localization of the gene for the human transcriptional repressor GCF . J Biol Chem . 267 . 3 . 1689–94 . Feb 1992 . 10.1016/S0021-9258(18)46000-0 . 1370479 . free .
2. Kageyama R, Pastan I . Molecular cloning and characterization of a human DNA binding factor that represses transcription . Cell . 59 . 5 . 815–25 . Jan 1990 . 2556218 . 10.1016/0092-8674(89)90605-3 . 21177070 .
3. Anthoni H, Zucchelli M, Matsson H, Muller-Myhsok B, Fransson I, Schumacher J, Massinen S, Onkamo P, Warnke A, Griesemann H, Hoffmann P, Nopola-Hemmi J, Lyytinen H, Schulte-Korne G, Kere J, Nothen MM, Peyrard-Janvid M . A locus on 2p12 containing the co-regulated MRPL19 and C2ORF3 genes is associated to dyslexia . Hum Mol Genet . 16 . 6 . 667–77 . Apr 2007 . 17309879 . 10.1093/hmg/ddm009 .
4. Web site: Entrez Gene: C2orf3 chromosome 2 open reading frame 3.