| Buttermilk Creek | |
| Pushpin Map: | Canada Southern Ontario |
| Pushpin Map Caption: | Location of the mouth of Buttermilk Creek in southern Ontario |
| Subdivision Type1: | Country |
| Subdivision Name1: | Canada |
| Subdivision Type2: | Province |
| Subdivision Name2: | Ontario |
| Subdivision Type3: | Region |
| Subdivision Name3: | Southwestern Ontario |
| Subdivision Type4: | County |
| Subdivision Name4: | Lambton |
| Subdivision Type5: | Town |
| Subdivision Name5: | Petrolia |
| Length: | 5km (03miles) |
| Source1: | Field |
| Source1 Coordinates: | 42.9025°N -82.1511°W |
| Source1 Elevation: | 203m (666feet) |
| Mouth: | Stonehouse Drain |
| Mouth Coordinates: | 42.8758°N -82.1822°W |
| Mouth Elevation: | 197m (646feet) |
| River System: | Great Lakes Basin |
Buttermilk Creek is a creek in the town of Petrolia, Lambton County, Ontario, Canada.
Buttermilk Creek begins in a field north of Petrolia and travels 5km (03miles) through the northwest of the town before reaching its mouth at the Stonehouse Drain in Lorne C Henderson Conservation Area. The Stonehouse Drain flows via Bear Creek and the North Sydenham and Sydenham Rivers into Lake St. Clair.
Buttermilk Creek is part of the St. Clair Region Conservation Authority's Lower Bear Watershed.[1]