Bolesatine Explained

Bolesatine is a glycoprotein isolated from the Rubroboletus satanas (Boletus satanas Lenz) mushroom which has a lectin function that is specific to the sugar binding site of D-galactose.[1] It is a monomeric protein with a compact globular structure and is thermostable. One tryptophan can be found in its primary sequence along with one disulfide bridge.

Bolesatine causes gastroenteritis in humans[2] and, at high enough concentrations, inhibits protein synthesis.[3] It does not inhibit protein synthesis directly. Instead, it acts as a phosphatase for nucleoside triphosphate, particularly for GTP.[4] At lower concentrations, it is a mitogen to human and rat T lymphocytes.[5] Studies have shown that at low concentrations, protein kinases C (PKC) are activated in vitro and in vero cells, leading to an increase in DNA synthesis activity.[6]

Effects of bolesatine poisoning

Other than the accumulation of toxins in human liver and organs, Bolesatine poisoning causes agglutination in human red blood cells and platelets at threshold concentrations. The following symptoms of hypertension and dizziness would be expected when affected. In severe cases, death may result.[7]

Notes and References

  1. Gachet. C.. Ennamany. R.. Kretz. O.. Ohlmann. P.. Krause. C.. Creppy. E. E.. Dirheimer. G.. Cazenave. J. P.. January 1996. Bolesatine induces agglutination of rat platelets and human erythrocytes and platelets in vitro. Human & Experimental Toxicology. 15. 1. 26–29. 10.1177/096032719601500105. 0960-3271. 8845205. 23915071 .
  2. Xenobiotica . January 1991 . 21 . 1 . 65–73 . Disposition of the toxic protein, bolesatine, in rats: its resistance to proteolytic enzymes. . Kretz . Olivier . Creppy . Edmond Ekue . Dirheimer . Guy . 2003368 . 10.3109/00498259109039451.
  3. Characterization of bolesatine, a toxic protein from the mushroom Boletus satanas Lenz and effects on kidney cells. . Olivier . Kretz . Edmond Ekue . Creppy . Guy . Dirheimer . Toxicology . February 1991 . 66 . 2 . 213–24 . 1707561 . 10.1016/0300-483x(91)90220-u.
  4. Ennamany. R.. Lavergne. J.P.. Reboud. J.P.. Dirheimer. G.. Creppy. E.E.. June 1995. Mode of action of bolesatine, a cytotoxic glycoprotein from Boletus satanas Lenz. Mechanistic approaches. Toxicology. 100. 1–3. 51–55. 10.1016/0300-483x(95)03058-n. 7624882. 0300-483X.
  5. International Journal of Biochemistry . 25 . 5 . May 1993 . 789–792 . Mitogenic activity and immunological properties of bolesatine, a lectin isolated from the mushroom Boletus satanas Lenz . Frederico . Licastro . Maria Cristina . Morini . Olivier . Kretz . Guy . Dirheimer . Edmond Ekue . Creppy . Fiorenzo . Stirpe . 10.1016/0020-711x(93)90366-m. 8349019 .
  6. Ennamany. R.. Marzetto. S.. Saboureau. D.. Creppy. E. E.. 1995-12-01. Lipid peroxidation induced by bolesatine, a toxin ofBoletus satanas: Implication in m5dC variation in Vero cells related to inhibition of cell growth. Cell Biology and Toxicology. en. 11. 6. 347–354. 10.1007/BF01305906. 8788210 . 20541517 . 1573-6822.
  7. Ennamany. R.. Bingen. A.. Creppy. E. E.. Kretz. O.. Gut. J. P.. Dubuisson. L.. Balabaud. C.. Bioulac Sage. P.. Kirn. A.. November 1998. Aspirin and heparin prevent hepatic blood stasis and thrombosis induced by the toxic glycoprotein Bolesatine in mice. Human & Experimental Toxicology. 17. 11. 620–624. 10.1177/096032719801701106. 0960-3271. 9865419. 43529948 .

This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Bolesatine".

Except where otherwise indicated, Everything.Explained.Today is © Copyright 2009-2024, A B Cryer, All Rights Reserved. Cookie policy.