| Bob Isbister | |
| Position: | Flying wing |
| Birth Date: | 9 June 1885 |
| Birth Place: | Hamilton, Ontario, Canada |
| Death Place: | Hamilton, Ontario, Canada |
| Playing Years1: | 1905–1915, 1919 |
| Playing Team1: | Hamilton Tigers |
| Career Highlights: | |
| Cfhof: | bob-isbister |
| Cfhofyear: | 1965 |
Robert "Big Bob" Isbister Sr. (June 9, 1885 – April 29, 1963) was a star football player in the Ontario Rugby Football Union 1905-1906 and then in the Big Four (IRFU) 1907-1915, 1919 for twelve seasons for the Hamilton Tigers. After retiring, he was a referee.
Isbister was born in Hamilton, Ontario, and died in Hamilton, Ontario. He was inducted into the Canadian Football Hall of Fame in 1965 and into the Canada's Sports Hall of Fame in 1975.
His son Bob Isbister Jr. was also an all-star Grey Cup champion Canadian football player, with the Toronto Argonauts.