Black Hole Quencher 1 Explained

Black Hole Quencher 1 (BHQ1) is an example of dark quencher, which is used to quench green and yellow dyes, such as 6-carboxyfluorescein (6-FAM), tetrachlorofluorescein (TET), and hexachlorofluorescein (HEX). The role of quenchers is to absorb energy from a fluorophore and to re-emit the energy in the form of either heat (dark quenchers) or visible light (fluorescent quenchers). The absorption range of BHQ1 is from 480 to 580 nm with maximum absorption at 534 nm.^{[1] [2] [3] [4]}

Notes and References

1. Didenko . V. V. . DNA probes using fluorescence resonance energy transfer (FRET): designs and applications . BioTechniques . November 2001 . 31 . 5 . 1106–1116, 1118, 1120–1121 . 10.2144/01315rv02 . 11730017 . 1941713 . 0736-6205.
2. Livak . K. J. . Flood . S. J. . Marmaro . J. . Giusti . W. . Deetz . K. . Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization . PCR Methods and Applications . June 1995 . 4 . 6 . 357–362 . 10.1101/gr.4.6.357 . 7580930 . 1054-9803. free .
3. Yeung . Anthony T. . Holloway . Brian P. . Adams . Pamela Scott . Shipley . Gregory L. . Evaluation of dual-labeled fluorescent DNA probe purity versus performance in real-time PCR . BioTechniques . February 2004 . 36 . 2 . 266–270, 272, 274–275 . 10.2144/04362RR01 . 14989091 . 0736-6205. free .
4. Tyagi . Sanjay . Kramer . Fred Russell . Molecular Beacons: Probes that Fluoresce upon Hybridization . Nature Biotechnology . March 1996 . 14 . 3 . 303–308 . 10.1038/nbt0396-303 . 9630890 . 27010207 . 30 August 2022 . en . 1546-1696.