| Bertholène | |
| Commune Status: | Commune |
| Image Coat Of Arms: | Blason de la ville de Bertholène (12).svg |
| Arrondissement: | Rodez |
| Canton: | Lot et Palanges |
| Insee: | 12026 |
| Postal Code: | 12310 |
| Mayor: | Christine Presne[1] |
| Term: | 2020 - 2026 |
| Coordinates: | 44.3956°N 2.7794°W |
| Elevation M: | 592 |
| Elevation Min M: | 533 |
| Elevation Max M: | 926 |
| Area Km2: | 46.96 |
Bertholène (in French pronounced as /bɛʁtɔlɛn/; Occitan (post 1500);: Bertolena) is a commune in the Aveyron department in southern France.