| Official Name: | Berkshire Lakes, Florida |
| Settlement Type: | Census-designated place |
| Pushpin Map: | Florida#USA |
| Pushpin Label: | Berkshire Lakes |
| Coordinates: | 26.1614°N -81.7278°W |
| Subdivision Type: | Country |
| Subdivision Type1: | State |
| Subdivision Name1: | Florida |
| Subdivision Type2: | County |
| Subdivision Name2: | Collier |
| Area Footnotes: | [1] |
| Area Total Sq Mi: | 0.78 |
| Area Land Sq Mi: | 0.66 |
| Area Water Sq Mi: | 0.12 |
| Area Total Km2: | 2.02 |
| Area Land Km2: | 1.70 |
| Area Water Km2: | 0.32 |
| Population As Of: | 2020 |
| Population Density Km2: | 1484.50 |
| Timezone: | Eastern (EST) |
| Utc Offset: | -5 |
| Timezone Dst: | EDT |
| Utc Offset Dst: | -4 |
| Elevation Ft: | 9 |
| Postal Code Type: | ZIP Code |
| Postal Code: | 34104 (Naples) |
| Area Code: | 239 |
| Blank Name: | FIPS code |
| Blank Info: | 12-05806 |
| Blank1 Name: | GNIS feature ID |
| Blank1 Info: | 2806002 |
| Population Total: | 2527 |
| Unit Pref: | Imperial |
| Population Density Sq Mi: | 3846.27 |
Berkshire Lakes is a census-designated place (CDP) in western Collier County, Florida, United States. It is 5miles east of Naples and is bordered to the northeast by Interstate 75.
Berkshire Lakes was first listed as a CDP prior to the 2020 census. The population was 2,527 at the 2020 census. It is a part of the Naples-Marco Island, Florida Metropolitan Statistical Area.