| Type: | cardinal |
| Honorific-Prefix: | His Eminence |
| Benedetto Giustiniani | |
| Church: | Catholic Church |
| Appointed: | 31 August 1620 |
| Ended: | 21 September 1631 |
| Predecessor: | Giovanni Evangelista Pallotta |
| Successor: | Francesco Maria Bourbon del Monte |
| Consecration: | 2 July 1612 |
| Consecrated By: | Pope Paul V |
| Birth Date: | 5 June 1554 |
| Birth Place: | Genoa, Italy |
| Cardinal: | 16 November 1586 |
| Death Place: | Rome |
| Buried: | Santa Maria sopra Minerva |
| Other Post: | Papal treasurer, Papal legate to Bologna |
Benedetto Giustiniani (5 June 1554 – 27 March 1621) was an Italian clergyman who was made a cardinal in the consistory of 16 November 1586 by Pope Sixtus V.
He participated in the papal conclaves of 1592 and 1621. From 1615 to 1620 he was bishop of the Sabina and from 1620 to 1621 of Porto. Either he or his brother Vincenzo commissioned the 1621-1629 painting of Saint John the Evangelist by Domenichino. His postmortem inventory contained 280 paintings.[1]