60S ribosomal protein L13 explained

60S ribosomal protein L13 is a protein that in humans is encoded by the RPL13 gene.^{[1] [2]}

Function

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13E family of ribosomal proteins. It is located in the cytoplasm. This gene is expressed at significantly higher levels in benign breast lesions than in breast carcinomas. Transcript variants derived from alternative splicing and/or alternative polyadenylation exist; these variants encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.^[2]

Interactions

RPL13 has been shown to interact with CDC5L.^[3]

Bbc1

Bbc1 (Mti1p) is a protein expressed in yeasts that is thought to associate with actin networks. Bbc1 is short for Bni1 synthetic lethal and Bee1 (las17) complex member.^[4] The alternate name, Mti1p, is short for Myosin tail region-interacting protein. Bbc1 is involved in cytoskeletal regulation during endocytosis. Budding yeast Bbc1 inhibits the activator of the Arp2/3 complex Las17 (WASp homolog).^[5] The protein also interacts with the tail of Myosin 1 proteins.^[6]

In fission yeast, Bbc1 is considered a WIP family cytoskeletal protein. Bbc1 localizes to actin cortical patches, cell division sites, the cell tip, and the cytosol. Cells with bbc1 gene deletion are viable.^[7] Bbc1 is affinity captured by the Nebulin-family actin filament anchoring protein Cyk3^[8] and the SMARCAD1 family ATP-dependent DNA helicase Fft3.^[9] Bbc1 competes with WIP homolog Vrp1 to bind the Myosin 1 tail to regulate actin assembly at endocytic sites.^[10]

Further reading

• Wool IG, Chan YL, Glück A . Structure and evolution of mammalian ribosomal proteins. . Biochem. Cell Biol. . 73 . 11–12 . 933–47 . 1996 . 8722009 . 10.1139/o95-101 .
• Adams SM, Helps NR, Sharp MG, Brammar WJ, Walker RA, Varley JM . Isolation and characterization of a novel gene with differential expression in benign and malignant human breast tumours . Hum. Mol. Genet. . 1 . 2 . 91–6 . 1993 . 1301162 . 10.1093/hmg/1.2.91 .
• Kato S, Sekine S, Oh SW, Kim NS, Umezawa Y, Abe N, Yokoyama-Kobayashi M, Aoki T . Construction of a human full-length cDNA bank . Gene . 150 . 2 . 243–50 . 1995 . 7821789 . 10.1016/0378-1119(94)90433-2 .
• Yaseen NR, Blobel G . Cloning and characterization of human karyopherin beta3 . Proc. Natl. Acad. Sci. U.S.A. . 94 . 9 . 4451–6 . 1997 . 9114010 . 20743 . 10.1073/pnas.94.9.4451 . 1997PNAS...94.4451Y . free .
• Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI . Directed proteomic analysis of the human nucleolus . Curr. Biol. . 12 . 1 . 1–11 . 2002 . 11790298 . 10.1016/S0960-9822(01)00650-9 . 14132033 . free . 2002CBio...12....1A .
• Bi W, Yan J, Stankiewicz P, Park SS, Walz K, Boerkoel CF, Potocki L, Shaffer LG, Devriendt K, Nowaczyk MJ, Inoue K, Lupski JR . Genes in a refined Smith-Magenis syndrome critical deletion interval on chromosome 17p11.2 and the syntenic region of the mouse . Genome Res. . 12 . 5 . 713–28 . 2002 . 11997338 . 186594 . 10.1101/gr.73702 .
• Andersen JS, Lam YW, Leung AK, Ong SE, Lyon CE, Lamond AI, Mann M . Nucleolar proteome dynamics . Nature . 433 . 7021 . 77–83 . 2005 . 15635413 . 10.1038/nature03207 . 2005Natur.433...77A . 4344740 .
• Kobayashi T, Sasaki Y, Oshima Y, Yamamoto H, Mita H, Suzuki H, Toyota M, Tokino T, Itoh F, Imai K, Shinomura Y . Activation of the ribosomal protein L13 gene in human gastrointestinal cancer . Int. J. Mol. Med. . 18 . 1 . 161–70 . 2006 . 16786168 . 10.3892/ijmm.18.1.161 . free .

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human 60S ribosomal protein L13

Notes and References

1. Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC . A map of 75 human ribosomal protein genes . Genome Res . 8 . 5 . 509–23 . August 1998 . 9582194 . 10.1101/gr.8.5.509 . free .
2. Web site: Entrez Gene: RPL13 ribosomal protein L13.
3. Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI . Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry . EMBO J. . 19 . 23 . 6569–81 . December 2000 . 11101529 . 305846 . 10.1093/emboj/19.23.6569 .
4. Web site: BBC1 SGD. www.yeastgenome.org. 2020-01-09.
5. Rodal AA, Manning AL, Goode BL, Drubin DG . Negative regulation of yeast WASp by two SH3 domain-containing proteins . Current Biology . 13 . 12 . 1000–8 . June 2003 . 12814545 . 10.1016/s0960-9822(03)00383-x . 11363803 . free . 2003CBio...13.1000R .
6. Mochida J, Yamamoto T, Fujimura-Kamada K, Tanaka K . The novel adaptor protein, Mti1p, and Vrp1p, a homolog of Wiskott-Aldrich syndrome protein-interacting protein (WIP), may antagonistically regulate type I myosins in Saccharomyces cerevisiae . Genetics . 160 . 3 . 923–34 . March 2002 . 10.1093/genetics/160.3.923 . 11901111 . 1462009 . 678661938 .
7. Web site: Pombase. www.pombase.org. 2020-01-09.
8. Roberts-Galbraith RH, Ohi MD, Ballif BA, Chen JS, McLeod I, McDonald WH, Gygi SP, Yates JR, Gould KL . 6 . Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site . Molecular Cell . 39 . 1 . 86–99 . July 2010 . 20603077 . 2916701 . 10.1016/j.molcel.2010.06.012 .
9. Lee J, Choi ES, Seo HD, Kang K, Gilmore JM, Florens L, Washburn MP, Choe J, Workman JL, Lee D . 6 . Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation . Nature Communications . 8 . 14527 . February 2017 . 28218250 . 5321744 . 10.1038/ncomms14527 . 2017NatCo...814527L .
10. MacQuarrie CD, Mangione MC, Carroll R, James M, Gould KL, Sirotkin V . S. pombe adaptor protein Bbc1 regulates localization of Wsp1 and Vrp1 during endocytic actin patch assembly . Journal of Cell Science . 132 . 17 . jcs233502 . September 2019 . 31391237 . 6771142 . 10.1242/jcs.233502 .