Bacteriophage pRNA explained

Bacteriophage pRNA
Symbol:Phage_pRNA
Rfam:RF00044
Rna Type:Gene
Tax Domain:Viruses

Bacteriophage pRNA is a ncRNA element. During replication of linear dsDNA viruses, the viral genome is packaged into the pre-formed viral procapsid. The packaging of DNA into the procapsid requires a molecular motor, which uses ATP as energy to accomplish the energetically unfavorable motion. In some bacteriophage, an RNA (pRNA) molecule is a vital component of this motor.[1] Structural analyses of the packaging motor have demonstrated that the pRNA molecule has fivefold symmetry when attached to the prohead.[2] [3] The pRNA is thought to be bound by the capsid connector protein.[4] Only the first 120 bases of the pRNA are essential for packing the viral DNA.[5] The pRNA is proposed to be composed of two domains, one corresponding to the first 120 bases and the second to the remaining 50 bases. Nuclear cleavage occurs in the single strand region linking these two domains.[6]

Notes and References

  1. Book: Guo P . Structure and function of phi29 hexameric RNA that drives the viral DNA packaging motor: review . Prog. Nucleic Acid Res. Mol. Biol. . 72 . 415–472 . 2002 . 12206459 . 10.1016/S0079-6603(02)72076-X . Progress in Nucleic Acid Research and Molecular Biology . 978-0-12-540072-5.
  2. Morais MC, Tao Y, Olson NH . Cryoelectron-microscopy image reconstruction of symmetry mismatches in bacteriophage phi29 . J. Struct. Biol. . 135 . 1 . 38–46 . 2001 . 11562164 . 5595366 . 10.1006/jsbi.2001.4379. etal.
  3. Simpson AA, Tao Y, Leiman PG . Structure of the bacteriophage phi29 DNA packaging motor . Nature . 408 . 6813 . 745–750 . 2000 . 11130079 . 10.1038/35047129. etal. 4151180 . 2000Natur.408..745S .
  4. Badasso MO, Leiman PG, Tao Y . Purification, crystallization and initial X-ray analysis of the head-tail connector of bacteriophage phi29 . Acta Crystallogr. D . 56 . Pt 9 . 1187–1190 . 2000 . 10957642 . 10.1107/S0907444900009239. etal.
  5. Guo PX, Erickson S, Anderson D . A small viral RNA is required for in vitro packaging of bacteriophage phi 29 DNA . Science . 236 . 4802 . 690–694 . 1987 . 3107124 . 10.1126/science.3107124. 1987Sci...236..690G .
  6. Bailey S, Wichitwechkarn J, Johnson D, Reilly BE, Anderson DL, Bodley JW . Phylogenetic analysis and secondary structure of the Bacillus subtilis bacteriophage RNA required for DNA packaging . J. Biol. Chem. . 265 . 36 . 22365–22370 . 1990 . 10.1016/S0021-9258(18)45714-6 . 2125049 . free .

This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Bacteriophage pRNA".

Except where otherwise indicated, Everything.Explained.Today is © Copyright 2009-2024, A B Cryer, All Rights Reserved. Cookie policy.