Transcription factor IIIB 50 kDa subunit explained

Transcription factor IIIB 50 kDa subunit (TFIIIB50) also known as b-related factor 2 (BRF-2) is a protein that in humans is encoded by the BRF2 gene.^{[1] [2] [3]}

Function

This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein.^[3]

Further reading

• Sutcliffe JE, Cairns CA, McLees A, etal . RNA polymerase III transcription factor IIIB is a target for repression by pocket proteins p107 and p130. . Mol. Cell. Biol. . 19 . 6 . 4255–61 . 1999 . 10330166 . 10.1128/mcb.19.6.4255. 104385 .
• Schramm L, Pendergrast PS, Sun Y, Hernandez N . Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters. . Genes Dev. . 14 . 20 . 2650–63 . 2000 . 11040218 . 10.1101/gad.836400 . 316990 .
• Teichmann M, Wang Z, Roeder RG . A stable complex of a novel transcription factor IIB- related factor, human TFIIIB50, and associated proteins mediate selective transcription by RNA polymerase III of genes with upstream promoter elements. . Proc. Natl. Acad. Sci. U.S.A. . 97 . 26 . 14200–5 . 2001 . 11121026 . 10.1073/pnas.97.26.14200 . 18895 . free .
• Hinkley CS, Hirsch HA, Gu L, etal . The small nuclear RNA-activating protein 190 Myb DNA binding domain stimulates TATA box-binding protein-TATA box recognition. . J. Biol. Chem. . 278 . 20 . 18649–57 . 2003 . 12621023 . 10.1074/jbc.M204247200 . free .
• Saxena A, Ma B, Schramm L, Hernandez N . Structure-function analysis of the human TFIIB-related factor II protein reveals an essential role for the C-terminal domain in RNA polymerase III transcription. . Mol. Cell. Biol. . 25 . 21 . 9406–18 . 2005 . 16227591 . 10.1128/MCB.25.21.9406-9418.2005 . 1265830 .

Notes and References

1. Yu Y, Zhang C, Zhou G, Wu S, Qu X, Wei H, Xing G, Dong C, Zhai Y, Wan J, Ouyang S, Li L, Zhang S, Zhou K, Zhang Y, Wu C, He F . Gene expression profiling in human fetal liver and identification of tissue- and developmental-stage-specific genes through compiled expression profiles and efficient cloning of full-length cDNAs . Genome Res . 11 . 8 . 1392–403 . Aug 2001 . 11483580 . 311073 . 10.1101/gr.175501 .
2. Cabart P, Murphy S . BRFU, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with TATA-binding protein . J Biol Chem . 276 . 46 . 43056–64 . Nov 2001 . 11564744 . 10.1074/jbc.M108515200 . free .
3. Web site: Entrez Gene: BRF2 BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like.